Autofluorescence: Difference between revisions

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Line 1: [[Image:PaperAutofluorescence.jpg\|thumb\|300px\|[[Micrograph]] of paper autofluorescing under [[ultraviolet]] illumination. The individual fibres in this sample are around 10 [[micrometre\|µmμm]] in diameter.]] '''Autofluorescence''' is the natural emission of light by biological structures such as [[mitochondria]] and [[lysosome]]s when they have absorbed light, and is used to distinguish the light originating from artificially added fluorescent markers ([[fluorophore]]s).<ref name=Monici-2005> {{cite journal Line 69: [[File:BananaSkin40X_Fluorescence.tif\|thumb\|220px\|right\|Autofluorescence in [[banana]] skin under different light conditions.]] [[Optical imaging]] systems that utilize [[multispectral]] imaging can reduce signal degradation caused by autofluorescence while adding enhanced [[multiplexing]] capabilities.<ref>{{cite journal \| url=https://dx.doi.org/10.1117/1.2032458 \| doi=10.1117/1.2032458 \| title=Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging \| date=2005 \| last1=Mansfield \| first1=James R. \| last2=Gossage \| first2=Kirk W. \| last3=Hoyt \| first3=Clifford C. \| last4=Levenson \| first4=Richard M. \| journal=Journal of Biomedical Optics \| volume=10 \| issue=4 \| page=041207 \| pmid=16178631 \| bibcode=2005JBO....10d1207M \| s2cid=35269802 \| doi-access=free }}</ref> The [[super resolution microscopy]] [[Vertico SMI\|SPDM]] revealed autofluorescent cellular objects which are not detectable under conventional fluorescence imaging conditions.<ref> Line 332: \| <ref> {{cite journal \|author1=Donaldson, ~~Llyod~~Lloyd \|author2=Williams, Nari \|date=February 2018 Line 394: ==See also== * [[Phosphorescence]]▼ * [[wikt:autoluminescence\|Autoluminescence]] ▲* [[Phosphorescence]] * [[Fluorescence in the life sciences]] ==References==