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Is the bug primarily related to salmon (bulk mode) or alevin (single-cell mode)?
It is related to salmon when used after umi tools deduplication.
Describe the bug
Once deduplicated the bams with umi_tools (and previously sorted by coordinates) salmon gives the following warning: WARNING: Detected suspicious pair ---
The names are different:
read1 : XXXXXXXXX-YYYY:YYYY
read2 : XXXXXXXXX-ZZZZ:ZZZZ
Is the bug primarily related to salmon (bulk mode) or alevin (single-cell mode)?
It is related to salmon when used after umi tools deduplication.
Describe the bug
Once deduplicated the bams with umi_tools (and previously sorted by coordinates) salmon gives the following warning: WARNING: Detected suspicious pair ---
The names are different:
read1 : XXXXXXXXX-YYYY:YYYY
read2 : XXXXXXXXX-ZZZZ:ZZZZ
To Reproduce
The used code is the following:
When I have treid to use the same sample without deduplication and sorting salmon works good.
Specifically, please provide at least the following information:
What I expect
I want to know how could I use salmon after umi_tools or to account for UMI deduplication
Desktop (please complete the following information):
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