DDX23
Vjerovatno ATP-ovisna RNK-helikaza DDX23 je enzim koji je kod ljudi kodiran genom DDX23.[5][6][7]
Ovaj gen kodira člana porodice proteina DEAD-boksa. Proteini izove kutije, okarakterizirani konzerviranim motivom Asp-Glu-Ala-Asp (DEAD), su pretpostavljene RNK-helikaze. Uključeni suu brojne ćelijske procese u promjeni sekundarne strukture RNK, kao što je iniciranje translacije, jedarna i mitohondrijska prerada, te sastavljanje ribosoma i splajsosoma. Na osnovu njihovih obrazaca distribucije, vjeruje se da su neki članovi ove porodice uključeni u embriogenezu, spermatogenezu i ćelijski rast i diobu. Protein kodiran ovim genom je komponenta kompleksa U5 snRNP; može olakšati konformacijske promjene u splajsosomu tokom prerade jedarne pre-iRNK. Alternativno prerađena varijanta transkripta pronađena je za ovaj gen, ali njena biološka valjanost nije utvrđena.[7]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 820 aminokiselina, a molekulska težina 95.583 Da.[8].
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAGELADKKD | RDASPSKEER | KRSRTPDRER | DRDRDRKSSP | SKDRKRHRSR | ||||
| DRRRGGSRSR | SRSRSKSAER | ERRHKERERD | KERDRNKKDR | DRDKDGHRRD | ||||
| KDRKRSSLSP | GRGKDFKSRK | DRDSKKDEED | EHGDKKPKAQ | PLSLEELLAK | ||||
| KKAEEEAEAK | PKFLSKAERE | AEALKRRQQE | VEERQRMLEE | ERKKRKQFQD | ||||
| LGRKMLEDPQ | ERERRERRER | MERETNGNED | EEGRQKIREE | KDKSKELHAI | ||||
| KERYLGGIKK | RRRTRHLNDR | KFVFEWDASE | DTSIDYNPLY | KERHQVQLLG | ||||
| RGFIAGIDLK | QQKREQSRFY | GDLMEKRRTL | EEKEQEEARL | RKLRKKEAKQ | ||||
| RWDDRHWSQK | KLDEMTDRDW | RIFREDYSIT | TKGGKIPNPI | RSWKDSSLPP | ||||
| HILEVIDKCG | YKEPTPIQRQ | AIPIGLQNRD | IIGVAETGSG | KTAAFLIPLL | ||||
| VWITTLPKID | RIEESDQGPY | AIILAPTREL | AQQIEEETIK | FGKPLGIRTV | ||||
| AVIGGISRED | QGFRLRMGCE | IVIATPGRLI | DVLENRYLVL | SRCTYVVLDE | ||||
| ADRMIDMGFE | PDVQKILEHM | PVSNQKPDTD | EAEDPEKMLA | NFESGKHKYR | ||||
| QTVMFTATMP | PAVERLARSY | LRRPAVVYIG | SAGKPHERVE | QKVFLMSESE | ||||
| KRKKLLAILE | QGFDPPIIIF | VNQKKGCDVL | AKSLEKMGYN | ACTLHGGKGQ | ||||
| EQREFALSNL | KAGAKDILVA | TDVAGRGIDI | QDVSMVVNYD | MAKNIEDYIH | ||||
| RIGRTGRAGK | SGVAITFLTK | EDSAVFYELK | QAILESPVSS | CPPELANHPD | ||||
| AQHKPGTILT | KKRREETIFA |
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000174243 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000003360 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Teigelkamp S, Mundt C, Achsel T, Will CL, Luhrmann R (Jan 1998). "The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p". RNA. 3 (11): 1313–26. PMC 1369570. PMID 9409622.
- ^ Laggerbauer B, Achsel T, Luhrmann R (maj 1998). "The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplices in vitro". Proc Natl Acad Sci U S A. 95 (8): 4188–92. doi:10.1073/pnas.95.8.4188. PMC 22463. PMID 9539711.
- ^ a b "Entrez Gene: DDX23 DEAD (Asp-Glu-Ala-Asp) box polypeptide 23".
- ^ "UniProt, Q9BUQ8". Pristupljeno 9. 8. 2021.
Dopunska literatura
[uredi | uredi izvor]- Achsel T, Ahrens K, Brahms H, et al. (1998). "The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein". Mol. Cell. Biol. 18 (11): 6756–66. doi:10.1128/mcb.18.11.6756. PMC 109259. PMID 9774689.
- Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4): 426–39. doi:10.1017/S1355838202021088. PMC 1370266. PMID 11991638.
- Irobi J, Nelis E, Verhoeven K, et al. (2002). "Mutation analysis of 12 candidate genes for distal hereditary motor neuropathy type II (distal HMN II) linked to 12q24.3". J. Peripher. Nerv. Syst. 7 (2): 87–95. doi:10.1046/j.1529-8027.2002.02014.x. PMID 12090300. S2CID 8453412.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
- Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
- Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.