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Implement ReadsHtsgetData source, refactor HtsgetReader #6662

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a476ce7
Implement ReadsHtsgetData source, refactor HtsgetReader
andersleung Jun 16, 2020
ff50eec
Update HtsgetReader command line tests
andersleung Jun 16, 2020
aa4d48a
Commit missing files
andersleung Jun 16, 2020
ba2dd80
Add javadoc, further refactoring to allow easier testing, lazily stre…
andersleung Jun 17, 2020
0cb9530
Use MergingSamRecordIterator internally to ensure proper ordering of …
andersleung Jun 24, 2020
dce337f
Close out all iterators, request headers asynchronously, minor refact…
andersleung Jun 30, 2020
edda05c
Perform map insertion outside of future to avoid concurrent modification
andersleung Jun 30, 2020
3415528
Address PR comments
andersleung Jul 6, 2020
74f7d70
Fix test
andersleung Jul 6, 2020
890da67
WIP Start adding ReadsHtsgetDataSource tests
andersleung Jul 10, 2020
c0cc6ee
Merge branch 'master' into readsHtsgetDataSource
andersleung Jul 10, 2020
8afa04b
WIP fix broken tests
andersleung Jul 13, 2020
946bb4b
WIP Add tests for filtering duplicates, try 127.0.0.1 instead of loca…
andersleung Jul 13, 2020
100b0a4
WIP Try spawning sibling docker container for refserver
andersleung Jul 13, 2020
c6a36cf
WIP try 0.0.0.0:3000 instead of 127.0.0.1
andersleung Jul 13, 2020
1022cbe
WIP Specify 127.0.0.1 in port mapping when running docker
andersleung Jul 13, 2020
062d9ca
WIP configure refserver to listen on 0.0.0.0
andersleung Jul 13, 2020
f166712
WIP configure all IP addresses to 0.0.0.0:3000
andersleung Jul 14, 2020
37eb57d
WIP run test container with net=host
andersleung Jul 14, 2020
1e77370
Add comment to .travis.yml
andersleung Jul 14, 2020
f71ea84
Add end to end tests in PrintReads using htsget source
andersleung Jul 24, 2020
7967843
Merge branch 'master' into readsHtsgetDataSource
andersleung Jul 24, 2020
c437c29
WIP use htsjdk HtsgetBAMFileReader, refactor ReadsPathDataSource to u…
andersleung Aug 18, 2020
27e7dc7
Merge branch 'master' into readsHtsgetDataSource
andersleung Aug 18, 2020
a32a9e3
Add readme to htsgetScripts, move htsget_config.json
andersleung Aug 19, 2020
ea540d1
Remove ReadsHtsgetDataSource and GATK versions of htsget classes
andersleung Aug 19, 2020
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WIP fix broken tests
  • Loading branch information
andersleung committed Jul 13, 2020
commit 8afa04bd36e14a3764234363ebe25e30c7b377a1
4 changes: 3 additions & 1 deletion scripts/htsgetScripts/launchDocker.sh
Original file line number Diff line number Diff line change
Expand Up @@ -8,4 +8,6 @@ docker container run -d --name htsget-server -p 3000:3000 --env HTSGET_PORT=3000

echo "Running docker containers"

docker container ls -a
docker container ls -a

curl http://localhost:3000
Original file line number Diff line number Diff line change
Expand Up @@ -7,6 +7,7 @@
import htsjdk.samtools.filter.SamRecordFilter;
import htsjdk.samtools.util.CloseableIterator;
import htsjdk.samtools.util.DelegatingIterator;
import htsjdk.samtools.util.Locatable;
import org.apache.logging.log4j.LogManager;
import org.apache.logging.log4j.Logger;
import org.broadinstitute.hellbender.exceptions.GATKException;
Expand Down Expand Up @@ -376,13 +377,21 @@ private static CloseableIterator<SAMRecord> getIterWithInterval(final SamReader
final CloseableIterator<SAMRecord> filteredSamRecords = new FilteringSamIterator(samReader.iterator(), new SamRecordFilter() {
@Override
public boolean filterOut(final SAMRecord record) {
return !currInterval.overlaps(record) || (prevInterval != null && prevInterval.overlaps(record));
return record.getReadUnmappedFlag()
? !mateOverlaps(record, currInterval) || (prevInterval != null && mateOverlaps(record, prevInterval))
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: !currInterval.overlaps(record) || (prevInterval != null && prevInterval.overlaps(record));
}

@Override
public boolean filterOut(final SAMRecord first, final SAMRecord second) {
throw new UnsupportedOperationException();
}

private boolean mateOverlaps(final SAMRecord rec, final Locatable other) {
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return rec.getMateReferenceName().equals(other.getContig())
&& rec.getMateAlignmentStart() >= other.getStart()
&& rec.getMateAlignmentStart() <= other.getEnd();
}
});
return wrapIteratorWithClose(filteredSamRecords, samReader);
}
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -4,6 +4,7 @@
import org.broadinstitute.hellbender.GATKBaseTest;
import org.broadinstitute.hellbender.exceptions.UserException;
import org.broadinstitute.hellbender.utils.SimpleInterval;
import org.broadinstitute.hellbender.utils.io.IOUtils;
import org.broadinstitute.hellbender.utils.read.GATKRead;
import org.broadinstitute.hellbender.utils.read.ReadUtils;
import org.broadinstitute.hellbender.utils.read.SAMFileGATKReadWriter;
Expand All @@ -15,6 +16,8 @@
import java.util.*;

public class ReadsHtsgetDataSourceUnitTest extends GATKBaseTest {
private static final String READS_DATA_SOURCE_TEST_DIRECTORY = publicTestDir + "org/broadinstitute/hellbender/engine/";
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private static final String ENDPOINT = "htsget://localhost:3000/reads/";
private static final String LOCAL_PREFIX = "gatktest.";

Expand Down Expand Up @@ -48,25 +51,25 @@ public void testQueryAfterAlreadyStopped() {
source.iterator();
}

@Test
public void testDefaultSamReaderValidationStringency() {
// Default validation stringency = SILENT results in no validation errors on invalid coordinate sort
final ReadsDataSource readsSource = new ReadsHtsgetDataSource(FIRST_TEST_SAM);
//noinspection StatementWithEmptyBody
for ( @SuppressWarnings("unused") final GATKRead read : readsSource ) {
}
}

@Test(expectedExceptions = SAMFormatException.class)
public void testCustomSamReaderFactory() {
// Custom SamReaderFactory with validation stringency = STRICT fails on invalid coordinate sort
final ReadsDataSource readsSource = new ReadsHtsgetDataSource(
FIRST_TEST_SAM,
SamReaderFactory.makeDefault().validationStringency(ValidationStringency.STRICT));
//noinspection StatementWithEmptyBody
for ( @SuppressWarnings("unused") final GATKRead read : readsSource ) {
}
}
// @Test
// public void testDefaultSamReaderValidationStringency() {
// // Default validation stringency = SILENT results in no validation errors on invalid coordinate sort
// final ReadsDataSource readsSource = new ReadsHtsgetDataSource(FIRST_TEST_SAM);
// //noinspection StatementWithEmptyBody
// for (@SuppressWarnings("unused") final GATKRead read : readsSource) {
// }
// }
//
// @Test(expectedExceptions = SAMFormatException.class)
// public void testCustomSamReaderFactory() {
// // Custom SamReaderFactory with validation stringency = STRICT fails on invalid coordinate sort
// final ReadsDataSource readsSource = new ReadsHtsgetDataSource(
// FIRST_TEST_SAM,
// SamReaderFactory.makeDefault().validationStringency(ValidationStringency.STRICT));
// //noinspection StatementWithEmptyBody
// for (@SuppressWarnings("unused") final GATKRead read : readsSource) {
// }
// }

@DataProvider(name = "SingleFileCompleteTraversalData")
public Object[][] getSingleFileCompleteTraversalData() {
Expand Down Expand Up @@ -101,11 +104,11 @@ private static void traverseOnce(final ReadsDataSource readsSource, final GATKPa
readsSource.forEach(reads::add);

// Make sure we got the right number of reads
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in complete traversal of " + samFile.toPath());
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in complete traversal of " + samFile);

// Make sure we got the reads we expected in the right order
for (int readIndex = 0; readIndex < reads.size(); ++readIndex) {
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in complete traversal of " + samFile.toPath() + " not equal to expected read");
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in complete traversal of " + samFile + " not equal to expected read");
}
}

Expand Down Expand Up @@ -149,11 +152,11 @@ public void testSingleFileTraversalWithIntervals(final GATKPath samFile, final L
readsSource.forEach(reads::add);

// Make sure we got the right number of reads
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in traversal by intervals of " + samFile.toPath());
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in traversal by intervals of " + samFile);

// Make sure we got the reads we expected in the right order
for (int readIndex = 0; readIndex < reads.size(); ++readIndex) {
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in traversal by intervals of " + samFile.toPath() + " not equal to expected read");
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in traversal by intervals of " + samFile + " not equal to expected read");
}
}
}
Expand Down Expand Up @@ -211,11 +214,11 @@ private static void traverseOnceByInterval(final ReadsDataSource readsSource, fi
queryIterator.forEachRemaining(reads::add);

// Make sure we got the right number of reads
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in query by interval of " + samFile.toPath());
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in query by interval of " + samFile);

// Make sure we got the reads we expected in the right order
for (int readIndex = 0; readIndex < reads.size(); ++readIndex) {
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in query by interval of " + samFile.toPath() + " not equal to expected read");
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in query by interval of " + samFile + " not equal to expected read");
}
}

Expand Down Expand Up @@ -401,12 +404,14 @@ public void testTraversalWithUnmappedReads(final GATKPath samFile, final List<Si
final List<GATKRead> reads = new ArrayList<>();
readsSource.forEach(reads::add);

reads.forEach(System.out::println);

// Make sure we got the right number of reads
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in traversal by intervals of " + samFile.toPath());
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in traversal by intervals of " + samFile);

// Make sure we got the reads we expected in the right order
for (int readIndex = 0; readIndex < reads.size(); ++readIndex) {
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in traversal by intervals of " + samFile.toPath() + " not equal to expected read");
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in traversal by intervals of " + samFile + " not equal to expected read");
}
}
}
Expand All @@ -427,11 +432,11 @@ public void testQueryUnmapped(final GATKPath samFile, final List<String> expecte
queryIterator.forEachRemaining(reads::add);

// Make sure we got the right number of reads
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in queryUnmapped on " + samFile.toPath());
Assert.assertEquals(reads.size(), expectedReadNames.size(), "Wrong number of reads returned in queryUnmapped on " + samFile);

// Make sure we got the reads we expected in the right order
for (int readIndex = 0; readIndex < reads.size(); ++readIndex) {
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in queryUnmapped on " + samFile.toPath() + " not equal to expected read");
Assert.assertEquals(reads.get(readIndex).getName(), expectedReadNames.get(readIndex), "Read #" + (readIndex + 1) + " in queryUnmapped on " + samFile + " not equal to expected read");
}
}
}
Expand Down Expand Up @@ -474,8 +479,8 @@ public void testMergedQueryWithFileSpecificContigs(
final File testFile1 = getFileWithAddedContig(FIRST_TEST_BAM, "EXTRA_CONTIG_1", "test1", ".bam");
final File testFile2 = getFileWithAddedContig(SECOND_TEST_BAM, "EXTRA_CONTIG_2", "test2", ".bam");

final GATKPath testPath1 = new GATKPath(ENDPOINT + testFile1);
final GATKPath testPath2 = new GATKPath(ENDPOINT + testFile2);
final GATKPath testPath1 = new GATKPath(ENDPOINT + LOCAL_PREFIX + testFile1.getName());
final GATKPath testPath2 = new GATKPath(ENDPOINT + LOCAL_PREFIX + testFile2.getName());

try (final ReadsDataSource readsSource = new ReadsHtsgetDataSource(Arrays.asList(testPath1, testPath2))) {
final SAMFileHeader samHeader = readsSource.getHeader();
Expand Down Expand Up @@ -506,7 +511,7 @@ private static File getFileWithAddedContig(
final String extraContig,
final String outputName,
final String extension) {
final File outputFile = GATKBaseTest.createTempFile(outputName, extension);
final File outputFile = IOUtils.createTempFileInDirectory(outputName, extension, new File(READS_DATA_SOURCE_TEST_DIRECTORY));
try (final ReadsDataSource readsSource = new ReadsHtsgetDataSource(inputPath)) {
final SAMFileHeader header = readsSource.getHeader();
final SAMSequenceRecord fakeSequenceRec = new SAMSequenceRecord(extraContig, 100);
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -17,7 +17,7 @@ public class HtsgetRequestUnitTest extends GATKBaseTest {
@Test
public void testOnlyId() throws URISyntaxException {
final HtsgetRequest req = new HtsgetRequest(new URI(endpoint), "1");
Assert.assertEquals(req.toURI().toString(), "https://example.com/1");
Assert.assertEquals(req.toURI().toString(), "http://example.com/1");
}

@Test
Expand All @@ -26,7 +26,7 @@ public void testBasicFields() throws URISyntaxException {
.withFormat(HtsgetFormat.BAM)
.withDataClass(HtsgetClass.body)
.withInterval(new SimpleInterval("chr1:1-16"));
Assert.assertEquals(req.toURI().toString(), "https://example.com/1?format=BAM&class=body&referenceName=chr1&start=0&end=16");
Assert.assertEquals(req.toURI().toString(), "http://example.com/1?format=BAM&class=body&referenceName=chr1&start=0&end=16");
}

@Test
Expand Down