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Description
【特許請求の範囲】
【請求項１】 流体試料から所望の物質を分離し、上記物質を濃縮して或る容積の溶離流体に入れるための微小流体装置において、上記装置は本体を備え、
ａ）上記試料を上記本体に導入し、続いて上記溶離流体を上記本体に導入するための少なくとも１つの入口ポートと、
ｂ）上記本体から上記試料と上記溶離流体を出すための少なくとも１つの出口ポートと、
ｃ）上記入口ポートと上記出口ポートを流体で連通するチャンバとを上記本体の中に形成し、上記出口ポートと上記入口ポートとは上記チャンバを通って上記本体から出る上記試料の連続的な流れと上記溶離流体の連続的な流れを可能するように配置され、
ｄ）上記チャンバの少なくとも１つの壁に一体に形成されると共に上記チャンバの中に伸びているコラムの配列を上記本体の中に形成して、上記試料が上記チャンバを通って流れるとき上記所望の物質を上記流体試料から捕獲し、続いて、上記溶離流体が上記チャンバを通って流れるとき上記捕獲された物質を上記溶離流体の中に放出し、上記コラムの各々は少なくとも２：１のアスペクト比を持っていることを特徴とする微小流体装置。
【請求項２】 流体試料を操作するための微小流体装置において、上記装置は、
ａ）本体を備え、上記本体は
i） チャンバと、
ii）上記チャンバと流体で連通するポートと、
iii）上記チャンバ内に延在するコラムの配列とを有し、
ｂ）上記コラムを振動させるための手段を備えていることを特徴とする微小流体装置。
【請求項３】 流体試料を操作するための微小流体装置において、上記装置は、
ａ）本体を備え、上記本体は
i） チャンバと、
ii）上記チャンバと流体で連通するポートと、
iii）上記チャンバ内に延在するコラムの配列とを有し、
ｂ）上記チャンバに超音波エネルギを伝達するための手段を備えていることを特徴とする微小流体装置。
【請求項４】 請求項１または２または３に記載の装置において、上記コラムを加熱するために少なくとも１つの加熱要素を備えていることを特徴とする装置。
【請求項５】 請求項４に記載の装置において、上記加熱要素は、上記チャンバの少なくとも１つの壁に結合された抵抗型加熱要素を備えていることを特徴とする装置。
【請求項６】 請求項４に記載の装置において、上記本体は電気的に伝導性があり、上記加熱要素は上記チャンバを形成している上記本体の少なくとも一部分に電位差を印加するための電極を備えていることを特徴とする装置。
【請求項７】 請求項１に記載の装置において、上記コラムを振動させるための手段を更に備えていることを特徴とする装置。
【請求項８】 請求項２または７に記載の装置において、上記コラムを振動させるための上記手段は、超音波ホーンを備えていることを特徴とする装置。
【請求項９】 請求項２または７に記載の装置において、上記コラムを振動させるための上記手段は、上記本体に結合された圧電性物質を備えていることを特徴とする装置。
【請求項１０】 請求項２または７に記載の装置において、上記コラムを振動させるための上記手段は、上記コラムを共振周波数で振動させることを特徴とする装置。
【請求項１１】 請求項１または２または３に記載の装置において、上記コラムの表面を帯電させるための電極を更に備えていることを特徴とする装置。
【請求項１２】 請求項１または２または３に記載の装置において、上記コラムの各々は少なくとも４：１のアスペクト比を有していることを特徴とする装置。
【請求項１３】 請求項１または２または３に記載の装置において、上記コラムの各々は、少なくとも４：１の断面の長さと幅の比を有していることを特徴とする装置。
【請求項１４】 請求項１または２または３に記載の装置において、上記装置は上記配列内に少なくとも１,０００個のコラムを含んでいることを特徴とする装置。
【請求項１５】 請求項１または２または３に記載の装置において、上記コラムは上記チャンバの底部壁と一体に形成されていると共に上記底部壁から略垂直に延在し、上記コラムの各々は上記チャンバの深さと実質的に等しい高さを有していることを特徴とする装置。
【請求項１６】 請求項１または２または３に記載の装置において、上記コラムは列になして配置され、上記各列のコラムは実質的に均一な中心と中心の間隔を有し、上記各列のコラムが、少なくとも１つの隣接する列のコラムから、上記隣接する列のコラムの中心と中心の間隔の半分よりも大きな距離または小さい距離だけオフセットされるように、上記各列のコラムは配置されていることを特徴とする装置。
【請求項１７】 請求項１または２または３に記載の装置において、各列のコラムが少なくとも２つ前の列または後の列のコラムと一直線上に並ばないように、上記列が配置されていることを特徴とする装置。
【請求項１８】 請求項１または２または３に記載の装置において、上記試料中の核酸を捕獲するために、上記コラムの表面は二酸化けい素を備えていることを特徴とする装置。
【請求項１９】 請求項２または３に記載の装置において、上記コラムの各々は少なくとも２：１のアスペクト比を有すると共に、上記コラムの表面は上記試料中の所望の物質に結合できることを特徴とする装置。
【請求項２０】 請求項１または２または３に記載の装置において、上記コラムの各々は少なくとも１つの特定の分析物捕獲表面を有していることを特徴とする装置。
【請求項２１】 請求項２０に記載の装置において、配位子が上記コラムに取付けられて上記特定の分析物捕獲表面を形成していることを特徴とする装置。
【請求項２２】 請求項２０に記載の装置において、上記特定の分析物捕獲表面は、上記試料中の標的ヌクレオチドシーケンスと相補する特定のヌクレオチドシーケンスを備えていることを特徴とする装置。
【請求項２３】 請求項１または２または３に記載の装置において、上記コラムの表面は、上記試料中の分析物に対して結合親和力を有する物質で被覆されていることを特徴とする装置。
【請求項２４】 請求項２３に記載の装置において、上記物質は、シリコンと、二酸化けい素と、ポリマーと、ポリアミドと、核酸と、金属と、ポリペプチドと、プロテインと、多糖類とからなるグループから選択されることを特徴とする装置。
【請求項２５】 請求項３に記載の装置において、上記チャンバに上記超音波エネルギーを伝えるための手段は、上記本体に接続された超音波ホーンを備えていることを特徴とする装置。
【請求項２６】 請求項３に記載の装置において、上記上記チャンバに上記超音波エネルギーを伝えるための手段は、上記本体に接続された圧電性物質を備えていることを特徴とする装置。
【請求項２７】 請求項２または３に記載の装置において、上記本体は少なくとも１つの入口ポートと少なくとも１つの出口ポートとを含んで、連続流体が上記チャンバを通って流れることを特徴とする装置。
【請求項２８】 請求項１または２７に記載の装置において、上記装置は上記流体試料のための前処理サイトを有するカートリッジと結合し、且つ、上記装置は上記カートリッジに挿入されて上記入口ポートが上記前処理サイトと流体で連通するように作られていることを特徴とする装置。
【請求項２９】 請求項１または２７に記載の装置において、上記装置は溶離物質のための後処理サイトを有するカートリッジと結合し、且つ、上記装置は上記カートリッジに挿入されて上記出口ポートが上記後処理サイトと流体で連通するように作られていることを特徴とする装置。
【請求項３０】 流体試料から所望の物質を分離し、上記物質を濃縮して或る容積の溶離流体に入れるための装置において、
ａ）本体を備えて、上記本体は
ｉ）チャンバと、
ii）上記試料を上記チャンバに導入し、続いて上記溶離流体を上記チャンバに導入するための少なくとも１つの入口ポートと、
iii）上記チャンバから上記試料と上記溶離流体を出すための少なくとも１つの出口ポートとを有し、上記出口ポートと上記入口ポートとは上記チャンバを通る上記試料の連続的な流れと上記溶離流体の連続的な流れを可能にするように配置され、
ｂ）上記チャンバ内に入れられた少なくとも１つの固形支持体を備えて、上記試料が上記チャンバを通って連続的に流れるとき上記試料から上記物質を捕獲し、上記固形支持体は、ビードとファイバと膜とグラスウールとフィルタペーパーとゲルとから成るグループから選択され、
ｃ）上記溶離流体が上記チャンバを通って流れるとき上記チャンバを加熱するためのヒーターを備えて、これによって上記固形支持体を上記溶離流体に捕獲された物質を放出させることを特徴とする装置。
【請求項３１】 流体試料から所望の物質を分離し、上記物質を濃縮して或る容積の溶離流体に入れるための方法であって、チャンバを有する微小流帯装置を使用する方法において、
ａ）上記チャンバ内に延在する上記コラムの配列に上記所望の物質を結合させるために、上記チャンバを通って強制的かつ連続的に上記試料を流すステップを備え、上記チャンバを通って強制的に流される試料の容積と上記チャンバの最大収容容積との比は少なくとも２：１であり、
ｂ）上記チャンバを通って強制的に上記溶離流体を流すステップを備え、これによって上記コラムの表面から上記溶離流体に上記物質を放出させることを特徴とする方法。
【請求項３２】 チャンバと上記チャンバ内に延在するコラムの配列とを有する装置を使用して、流体試料から所望の物質を分離する方法において、
ａ）上記コラムの表面に上記所望の物質を結合させるために、上記チャンバを通って強制的に上記試料を流すステップと、
ｂ）i）上記チャンバを通って強制的に溶離流体を流すことにより、且つ
ii）上記溶離流体が上記チャンバ内に存在する間、上記コラムを振動させることにより、上記チャンバから上記物質を溶離させるステップとを備えていることを特徴とする方法。
【請求項３３】 チャンバと上記チャンバ内に延在するコラムの配列とを有する装置を使用して、流体試料を操作する方法において、
ａ）上記試料を強制的に上記チャンバ内に流すステップと、
ｂ）上記溶離流体が上記チャンバ内に存在する間、上記コラムを振動させるステップとを備えたことを特徴とする方法。
【請求項３４】 チャンバと上記チャンバ内に延在するコラムの配列とを有する装置を使用して、流体試料を操作する方法において、
ａ）上記試料を強制的に上記チャンバ内に流すステップと、
ｂ）上記溶離流体が上記チャンバ内に存在する間、超音波エネルギを上記チャンバ内に伝えるステップとを備えたことを特徴とする方法。
【請求項３５】 チャンバを有する装置を使用して、流体試料から所望の物質を分離するための方法において、
ａ）上記チャンバを通って強制的に上記試料を流すステップを備え、これによって、上記チャンバ内に配置された少なくとも１つの固形支持体に上記所望の物質を結合させ、上記チャンバを通って強制的に流される試料の容積と上記チャンバの最大収容容積との比は少なくとも２：１であり、上記チャンバを通って強制的に流される試料の容積は少なくとも０．５ｍｌであり、上記固形支持体はビードとファイバと膜とグラスウールとフィルタペーパーとゲルとから成るグループから選択され、
ｂ）上記チャンバを通って強制的に上記溶離流体を流す間に上記チャンバを加熱するステップを備え、これによって上記固形支持体から上記溶離流体に上記物質を放出させることを特徴とする方法。
【請求項３６】 請求項３３または３４に記載の方法において、
ａ）上記試料の中の所望の物質を上記コラムの表面に結合させるために、上記チャンバを通って上記試料を強制的に流すステップと、
ｂ）上記チャンバを通って強制的に溶離流体を流すステップとを更に備え、これによって上記所望の物質を上記コラム表面から上記溶離流体内に放出させることを特徴とする方法。
【請求項３７】 請求項３６に記載の方法において、上記チャンバを通って強制的に流される試料の容積と上記チャンバの最大収容容積との比は少なくとも２：１であることを特徴とする方法。
【請求項３８】 請求項３１または３２または３６に記載の方法において、上記溶離流体が上記チャンバ内に存在する間、上記コラムを加熱するステップをさらに備えていることを特徴とする方法。
【請求項３９】 請求項３８に記載の方法において、上記コラムは６０度Ｃから９５度Ｃの範囲の温度に加熱されることを特徴とする方法。
【請求項４０】 請求項３１または３２または３５または３６に記載の方法において、上記チャンバを通して強制的に試料を流すステップの後、且つ、上記溶離流体を上記チャンバに強いて流すステップの前に、ガスを上記チャンバに強いて流すステップをさらに備えていることを特徴とする方法。
【請求項４１】 請求項３１または３２または３５または３６に記載の方法において、上記チャンバを通って強制的に流される試料の容積と上記チャンバの最大収容容積との比は少なくとも１０：１であることを特徴とする方法。
【請求項４２】 請求項３１または３２または３６に記載の方法において、上記チャンバを通って強制的に流される試料の容積は少なくとも０．５ｍｌであることを特徴とする方法。
【請求項４３】 請求項３１または３２または３６に記載の方法において、上記物質を誘引あるいは溶離するために上記コラムの表面を帯電させるステップをさらに備えていることを特徴とする方法。
【請求項４４】 請求項３５に記載の方法において、上記チャンバは６０度Ｃから９５度Ｃの範囲の温度に加熱されることを特徴とする方法。
【請求項４５】 請求項３２または３３または３４に記載の方法において、上記コラムの各々は少なくとも２：１のアスペクト比を有していることを特徴とする方法。
【請求項４６】 請求項３１乃至３４のいずれか１つに記載の方法において、上記コラムの各々は少なくとも４：１の断面の長さと幅の比を有していることを特徴とする方法。
【請求項４７】 請求項３２または３３に記載の方法において、上記コラムは共振周波数で振動されることを特徴とする方法。
【請求項４８】 請求項３３または３４に記載の方法において、上記コラムの各々は少なくとも１つの特定の分析物捕獲表面を有し、上記コラムの表面に上記試料内の分析物を結合させるステップを更に備えていることを特徴とする方法。
【請求項４９】 流体を操作するための微小流体装置であって、
少なくとも第１流体と第２流体とをそれぞれ通す第１溝と第２溝とを有し、上記第１溝と上記第２溝はこれらの流体流の接触領域を提供する共通溝に集まり、上記溝の各々はその幅より大きな深さを有し、上記第１溝と第２溝に流れる流体は上記共通溝に流れ込んで、上記共通溝の中で並んで流れる少なくとも２つの垂直な流体シースを形成することを特徴とする微小流体装置。
【請求項５０】 流体を操作するための装置であって、
ａ）第１溝と、
ｂ）上記第１溝から分岐している第２溝と第３溝とを上記装置の中に形成して、上記第１溝に流れる流体を少なくとも２つの流体流に分離し、上記溝の各々はその幅より大きな深さを有することを特徴とする装置。
【請求項５１】 請求項４９に記載の装置において、上記共通溝に配置されたピラーを備えて上記流体流の安定性を増大させていることを特徴とする装置。
【請求項５２】 請求項４９に記載の装置において、上記第１溝と上記第２溝とは複数の共通溝に合流して、対応した複数の接触領域を上記流体流に対して提供し、上記共通溝の各々はその幅よりも大きな深さを有していることを特徴とする装置。
【請求項５３】 請求項４９に記載の装置において、上記共通溝は少なくとも１回１８０度回転をさせることを特徴とする装置。
【請求項５４】 請求項４９または５０に記載の装置において、上記溝の各々は少なくとも５：１の内部表面積と外面表面積の比を有することを特徴とする装置。
【請求項５５】 請求項４９または５０に記載の装置において、上記溝の各々は少なくとも１０：１の内部表面積と外面表面積の比を有することを特徴とする装置。
【請求項５６】 請求項４９または５０に記載の装置において、上記溝の各々は少なくとも２０：１の内部表面積と外面表面積の比を有することを特徴とする装置。
【請求項５７】 請求項４９または５０に記載の装置において、上記溝の各々は１０から１,０００マイクロメータの範囲の深さと、５から５０マイクロメータの範囲の幅とを有していることを特徴とする装置。
[Claims]
1. A microfluidic device for separating a desired substance from a fluid sample and concentrating said substance into a volume of an elution fluid, said apparatus comprising a body,
a) at least one inlet port for introducing the sample into the body and subsequently introducing the elution fluid into the body;
b) at least one outlet port for exiting the sample and the elution fluid from the body;
c) forming a chamber in the body that fluidly connects the inlet port and the outlet port, wherein the outlet port and the inlet port are a continuous flow of the sample out of the body through the chamber; And is arranged to allow a continuous flow of the eluting fluid,
d) forming in the body an array of columns integrally formed in at least one wall of the chamber and extending into the chamber so that the desired sample flows as the sample flows through the chamber; Capturing material from the fluid sample and subsequently releasing the captured material into the elution fluid as the elution fluid flows through the chamber, wherein each of the columns has an aspect ratio of at least 2: 1. A microfluidic device characterized by having:
2. A microfluidic device for manipulating a fluid sample, said device comprising:
a) comprising a body, wherein the body is
i) a chamber;
ii) a port in fluid communication with the chamber;
iii) an array of columns extending into the chamber;
b) A microfluidic device comprising means for vibrating the column.
3. A microfluidic device for manipulating a fluid sample, said device comprising:
a) comprising a body, wherein the body is
i) a chamber;
ii) a port in fluid communication with the chamber;
iii) an array of columns extending into the chamber;
b) A microfluidic device comprising means for transmitting ultrasonic energy to said chamber.
4. Apparatus according to claim 1, comprising at least one heating element for heating the column.
5. The apparatus according to claim 4, wherein said heating element comprises a resistive heating element coupled to at least one wall of said chamber.
6. The apparatus according to claim 4, wherein said body is electrically conductive and said heating element comprises an electrode for applying a potential difference to at least a portion of said body forming said chamber. An apparatus, comprising:
7. The apparatus of claim 1, further comprising means for oscillating said column.
8. The apparatus according to claim 2, wherein said means for oscillating said column comprises an ultrasonic horn.
9. The apparatus according to claim 2, wherein said means for oscillating said column comprises a piezoelectric material coupled to said body.
10. The apparatus according to claim 2, wherein said means for oscillating said column oscillates said column at a resonance frequency.
11. The apparatus according to claim 1, further comprising an electrode for charging a surface of the column.
12. Apparatus according to claim 1, 2 or 3, wherein each of said columns has an aspect ratio of at least 4: 1.
13. The apparatus of claim 1, 2 or 3, wherein each of the columns has a length to width ratio of at least 4: 1 in cross section.
14. The device according to claim 1, wherein the device comprises at least 1,000 columns in the array.
15. The apparatus of claim 1, 2 or 3, wherein the columns are formed integrally with and extend substantially perpendicularly from the bottom wall of the chamber, and each of the columns is An apparatus having a height substantially equal to the depth of the chamber.
16. The apparatus of claim 1, wherein the columns are arranged in rows, the columns of each row having a substantially uniform center-to-center spacing, The columns of each row are arranged such that the columns of the rows are offset from the columns of at least one adjacent row by a distance greater than or less than half the center-to-center spacing of the columns of the adjacent rows. An apparatus characterized in that:
17. The apparatus according to claim 1, wherein the rows are arranged such that the columns of each row are not aligned with the columns of at least two preceding or following rows. An apparatus characterized in that:
18. The device according to claim 1, wherein the surface of the column is provided with silicon dioxide for capturing nucleic acids in the sample.
19. The apparatus according to claim 2, wherein each of the columns has an aspect ratio of at least 2: 1 and the surface of the column is capable of binding to a desired substance in the sample. Equipment to do.
20. Apparatus according to claim 1, 2 or 3, wherein each of the columns has at least one specific analyte capture surface.
21. The apparatus of claim 20, wherein a ligand is attached to said column to form said specific analyte capture surface.
22. The device of claim 20, wherein the specific analyte capture surface comprises a specific nucleotide sequence that is complementary to a target nucleotide sequence in the sample.
23. The device according to claim 1, wherein the surface of the column is coated with a substance having a binding affinity for an analyte in the sample.
24. The device according to claim 23, wherein the substance comprises silicon, silicon dioxide, a polymer, a polyamide, a nucleic acid, a metal, a polypeptide, a protein, and a polysaccharide. An apparatus selected from a group.
25. The apparatus according to claim 3, wherein the means for transmitting the ultrasonic energy to the chamber comprises an ultrasonic horn connected to the body.
26. The apparatus according to claim 3, wherein the means for transmitting the ultrasonic energy to the chamber comprises a piezoelectric material connected to the body.
27. The apparatus according to claim 2 or 3, wherein the body includes at least one inlet port and at least one outlet port, and wherein a continuous fluid flows through the chamber. .
28. The device according to claim 1 or 27, wherein the device is coupled to a cartridge having a pretreatment site for the fluid sample, and the device is inserted into the cartridge and the inlet port is closed. An apparatus characterized in that it is adapted to be in fluid communication with the pretreatment site.
29. The apparatus according to claim 1 or 27, wherein said apparatus is coupled to a cartridge having a post-processing site for eluent, and said apparatus is inserted into said cartridge and said outlet port is connected to said cartridge. An apparatus characterized in that it is adapted to be in fluid communication with a post-treatment site.
30. An apparatus for separating a desired substance from a fluid sample and concentrating said substance into a volume of an eluent fluid.
a) comprising a body, said body comprising: i) a chamber;
ii) at least one inlet port for introducing the sample into the chamber and subsequently introducing the elution fluid into the chamber;
iii) having at least one outlet port for exiting the sample and the eluant fluid from the chamber, wherein the outlet port and the inlet port define a continuous flow of the sample through the chamber and a flow of the eluant fluid. Arranged to allow continuous flow,
b) including at least one solid support contained within the chamber for capturing the substance from the sample as the sample continuously flows through the chamber, the solid support comprising a bead and a fiber; And membranes, glass wool, filter paper and gels,
c) An apparatus comprising a heater for heating the chamber as the elution fluid flows through the chamber, thereby causing the solid support to release substances trapped in the elution fluid.
31. A method for separating a desired substance from a fluid sample and concentrating the substance into a volume of eluent fluid, the method comprising using a microfluidic device having a chamber.
a) forcibly and continuously flowing the sample through the chamber to bind the desired substance to the array of columns extending into the chamber; The ratio of the volume of the sample flowing into the chamber to the maximum capacity of the chamber is at least 2: 1;
b) forcing the elution fluid to flow through the chamber, thereby releasing the substance from the surface of the column to the elution fluid.
32. A method for separating a desired substance from a fluid sample using an apparatus having a chamber and an array of columns extending into the chamber, the method comprising:
a) forcing the sample through the chamber to bind the desired substance to the surface of the column;
b) i) by forcing an elution fluid through the chamber, and
ii) oscillating the column while the elution fluid is in the chamber to elute the substance from the chamber.
33. A method of manipulating a fluid sample using an apparatus having a chamber and an array of columns extending into the chamber, the method comprising:
a) forcing the sample into the chamber;
b) vibrating the column while the eluting fluid is in the chamber.
34. A method of manipulating a fluid sample using an apparatus having a chamber and an array of columns extending into the chamber, the method comprising:
a) forcing the sample into the chamber;
b) transmitting ultrasonic energy into said chamber while said eluting fluid is present in said chamber.
35. A method for separating a desired substance from a fluid sample using an apparatus having a chamber, comprising:
a) forcing the sample to flow through the chamber, thereby binding the desired substance to at least one solid support disposed within the chamber, and forcing through the chamber. The ratio of the volume of the sample flowed through the chamber to the maximum storage volume of the chamber is at least 2: 1, the volume of the sample forced through the chamber is at least 0.5 ml, and the solid support is Selected from the group consisting of beads, fibers, membranes, glass wool, filter papers and gels,
b) heating the chamber while forcing the elution fluid through the chamber, thereby releasing the substance from the solid support into the elution fluid.
36. The method according to claim 33 or claim 34, wherein
a) forcing the sample through the chamber to bind a desired substance in the sample to the surface of the column;
b) forcing a flow of the elution fluid through the chamber, whereby the desired substance is released from the column surface into the elution fluid.
37. The method of claim 36, wherein the ratio of the volume of the sample forced through the chamber to the maximum capacity of the chamber is at least 2: 1. .
38. The method of claim 31 or 32 or 36, further comprising the step of heating the column while the eluting fluid is in the chamber.
39. The method according to claim 38, wherein the column is heated to a temperature in the range of 60 degrees Celsius to 95 degrees Celsius.
40. The method of claim 31 or 32 or 35 or 36, wherein after the step of forcing a sample to flow through the chamber and before the step of forcing the elution fluid through the chamber. Further comprising the step of forcing flow through the chamber.
41. The method of claim 31 or 32 or 35 or 36, wherein the ratio of the volume of the sample forced through the chamber to the maximum capacity of the chamber is at least 10: 1. A method comprising:
42. The method according to claim 31, wherein the volume of the sample forced through the chamber is at least 0.5 ml.
43. A method according to claim 31 or 32 or 36, further comprising the step of charging the surface of the column to attract or elute the substance.
44. The method of claim 35, wherein the chamber is heated to a temperature in a range from 60 degrees Celsius to 95 degrees Celsius.
45. The method of claim 32 or claim 33, wherein each of the columns has an aspect ratio of at least 2: 1.
46. The method according to claim 31, wherein each of the columns has a length to width ratio of at least 4: 1 in cross section.
47. A method according to claim 32 or claim 33, wherein the column is oscillated at a resonance frequency.
48. The method of claim 33 or claim 34, wherein each of the columns has at least one specific analyte capture surface, and the step of binding an analyte in the sample to the surface of the column. A method, further comprising:
49. A microfluidic device for manipulating a fluid, comprising:
A first groove and a second groove for passing at least a first fluid and a second fluid, respectively, wherein the first groove and the second groove gather in a common groove providing a contact area for these fluid flows; Each of the grooves has a depth greater than its width, and the fluid flowing in the first and second grooves flows into the common groove to form at least two vertical fluid sheaths flowing side by side in the common groove. A microfluidic device characterized by being formed.
50. An apparatus for manipulating a fluid, comprising:
a) a first groove;
b) forming a second groove and a third groove branching from the first groove in the device to separate the fluid flowing in the first groove into at least two fluid streams, each of the grooves; Is a device having a depth greater than its width.
51. The apparatus according to claim 49, further comprising pillars disposed in said common groove to increase the stability of said fluid flow.
52. The apparatus of claim 49, wherein the first groove and the second groove merge into a plurality of common grooves to provide a corresponding plurality of contact areas for the fluid flow; Apparatus, wherein each of said common grooves has a depth greater than its width.
53. The apparatus according to claim 49, wherein the common groove rotates at least once by 180 degrees.
54. The device of claim 49 or claim 50, wherein each of the grooves has a ratio of internal surface area to external surface area of at least 5: 1.
55. The apparatus of claim 49 or claim 50, wherein each of the grooves has a ratio of internal surface area to external surface area of at least 10: 1.
56. The device of claim 49 or claim 50, wherein each of the grooves has a ratio of internal surface area to external surface area of at least 20: 1.
57. The device according to claim 49 or 50, wherein each of the grooves has a depth in the range of 10 to 1,000 micrometers and a width in the range of 5 to 50 micrometers. An apparatus characterized by the above.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/910,434 US6368871B1 (en) | 1997-08-13 | 1997-08-13 | Non-planar microstructures for manipulation of fluid samples |
| US08/910,434 | 1997-08-13 | ||
| US11545498A | 1998-07-14 | 1998-07-14 | |
| US09/115,454 | 1998-07-14 | ||
| PCT/US1998/016870 WO1999009042A2 (en) | 1997-08-13 | 1998-08-13 | Microstructures for the manipulation of fluid samples |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2001515216A JP2001515216A (en) | 2001-09-18 |
| JP2001515216A5 true JP2001515216A5 (en) | 2006-01-05 |
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ID=26813216
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000509721A Pending JP2001515216A (en) | 1997-08-13 | 1998-08-13 | Microstructure for manipulating fluid samples |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1003759A2 (en) |
| JP (1) | JP2001515216A (en) |
| AU (1) | AU745989B2 (en) |
| CA (1) | CA2301309A1 (en) |
| WO (1) | WO1999009042A2 (en) |
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