JP3240326B2 - Method for producing microbial cells SKS - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a useful microorganism SK.
S relates to a process for the preparation of.

[0002]

2. Description of the Related Art The microbial cells SKS of the present invention have not been heretofore existed because they are newly produced. This microbial cell SKS has a property of extremely activating other useful microbial cells, and a microbial cell having such a property has never existed or is very rarely present. is there.

[0003]

The microbial cells SKS
The object of the present invention is to provide a useful microorganism cell SKS in a large amount by providing the method itself and a method for producing the same, and to cope with various uses.

[0004]

[0005]

Means for Solving the Problems The invention of the first aspect of this application is a method for producing a microbial cell SKS, which is a SKS-1 bacterium FERM B belonging to Gram-positive spore-forming spores (mesophilic).
P-3692 , SKS-2 bacterium FERM BP-3693 belonging to gram-positive spore-forming bacterium (thermophilic), SKS-3 bacterium FE belonging to catalase-positive gram-positive spore-free bacillus
RM BP-3694 , SK belonging to actinomycetes (thermophilic)
S-4 bacteria FERM BP-3695 , actinomycetes (medium temperature)
Bacterium FERM BP-3696 belonging to SKS-6, SKS-6 FE belonging to catalase-positive Gram-positive cocci
RM BP-3697 , a SKS-7 bacterium belonging to intestinal bacteria
FERM BP-3698 , SK belonging to fungi (mold)
S-8 bacteria FERM BP-3699 and SKS-9 bacteria FERM BP-370 belonging to anaerobic spore-forming bacilli
0 were cultured separately respectively, in which is prepared by mixing them culture.

[0006]

[Action] Each of the bacteria constituting the front Symbol microbial cells SKS is an international deposit to the Agency Fermentation Research Institute, each of the accession numbers are as follows. SKS-1 bacteria belonging to Gram-positive spore-forming bacteria (medium temperature) are micro-engineered articles No. 3692 (FERM BP-3692) , and SKS-2 bacteria belonging to Gram-positive spore-forming bacteria (thermophilic ) are micro-engineered. Kenzo No. 3693 (FERM BP-3693) , a SKS- belonging to catalase-positive, gram-positive aporous spores.
The three bacteria are described in Micromachin Kenjo No. 3694 (FERMBP-369).
4) SKS-4 bacterium belonging to actinomycetes (thermophilic) is micromanipulated by No. 3695 (FERM BP-3695) , and SKS-5 bacterium belonging to actinomycetes (medium temperature) is micromanipulated by No. 3696
No. (FERM BP-3696) , SKS-6 belonging to catalase-positive, Gram-positive cocci is described in
No. 7 (FERM BP-3697), SKS -7 bacteria belonging to the intestinal bacteria fine Industrial Research Article No. 3698 (FERM BP
-3698), SKS-8 bacteria fine Industrial Research Article No. 3699 belonging to fungi (molds) (FERM BP-3699), and the knitted anaerobic SKS-9 bacteria belonging to the spore-forming bacillus of the first fine-Industrial Research Article No. 3700 (FERM BP-3700) .

[0007] The SKS-1 to SKS-9 bacteria described above are:
It is produced from the foot of Oyama in Tottori Prefecture. The microbial cells SKS obtained by mixing these nine bacteria as described above activate other useful microorganisms as a result of long-term studies by the inventors, and the present invention has been accomplished. It is a thing. The microbial cell SKS emits antibiotics, various vitamins, growth hormones, and organic substances as its metabolites, which are considered to be useful nutrients, and bring about useful microorganisms.

[0008]

EXAMPLES Examples of the method for producing microbial cells SKS of the present invention will be described. . First, SKS-1 bacteria to SKS
Each culture of -9 bacteria will be described in detail below.

[0009] The SKS-1 bacterium is an aerobic bacterium belonging to Gram-positive spore bacilli (mesophilic). This fungus has 12
It can be produced by culturing on a tryptic soy agar medium (Eiken Chemical Co., E-MC11) sterilized at 1 ° C. for 15 minutes at 30 ° C. for 3 to 5 days. The pH of the medium is 7.3. The composition of the medium is 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride, and 15 g of agar. The SKS-1 bacteria can be stored by a freeze-drying method (stored at around + 5 ° C.). In this case, the composition of the protective agent is 11
It is a 10% skim milk aqueous solution without pH adjustment sterilized at 0 ° C. for 10 minutes. To restore this, use sterile water at room temperature.

[0010] SKS-2 bacteria are aerobic bacteria belonging to Gram-positive spore-forming bacilli (thermophilic). This fungus has 12
It can be produced by culturing on a tryptic soy agar medium (Eiken Chemical Co., Ltd., E-MC11) sterilized at 1 ° C. for 15 minutes at 55 ° C. for 1 to 3 days. The pH of the medium is 7.3. The composition of the medium is 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride, and 15 g of agar. The SKS-2 bacteria can be stored by a freeze-drying method (stored at around + 5 ° C.). In this case, the composition of the protective agent is 11
It is a 10% skim milk aqueous solution without pH adjustment sterilized at 0 ° C. for 10 minutes. To restore this, use sterile water at room temperature.

[0011] The SKS-3 bacterium is an aerobic bacterium belonging to catalase-positive gram-positive spore-free bacilli. This fungus
Tryptosoy agar medium (Eiken Chemical Co., E-MC11) sterilized at 121 ° C for 15 minutes at 30 ° C for 3 to 5 minutes.
It can be produced by culturing for days. The pH of the medium is 7.3.
The composition of the medium is composed of 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride, and 15 g of agar. The SKS-2 bacterium can be stored by a freeze-drying method (stored at around + 5 ° C.).
It is a 10% skim milk aqueous solution without pH adjustment sterilized at 10 ° C. for 10 minutes. To restore this, use sterile water at room temperature.

[0012] SKS-4 bacteria are aerobic bacteria belonging to actinomycetes (thermophilic). This bacterium was used in a tryptic soy agar medium (Eiken Chemical Co., E.
-MC11) and cultured at 55 ° C for 3 to 5 days. The pH of the medium is 7.3. The composition of the medium is 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride, and 15 g of agar. SKS
-4 bacteria can be stored by a freeze-drying method (stored at around + 5 ° C.). In this case, the composition of the protective agent is a 10% aqueous solution of skim milk without pH adjustment sterilized at 110 ° C. for 10 minutes.
To restore this, use sterile water at room temperature.

[0013] SKS-5 bacteria are aerobic bacteria belonging to actinomycetes (mesophilic). This bacterium was used in a tryptic soy agar medium (Eiken Chemical Co., E.
-MC11) and cultured at 30 ° C for 5 to 7 days. The pH of the medium is 7.3. The composition of the medium is 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride, and 15 g of agar. SKS
The -5 bacteria can be stored by a freeze-drying method (stored at around + 5 ° C.). In this case, the composition of the protective agent is a 10% aqueous solution of skim milk without pH adjustment sterilized at 110 ° C. for 10 minutes.
To restore this, use sterile water at room temperature.

[0014] The SKS-6 bacterium is an aerobic bacterium belonging to catalase-positive gram-positive cocci. This fungus is 121
It can be produced by culturing on a tryptic soy agar medium (Eiken Chemical Co., E-MC11) sterilized at 15 ° C. for 15 minutes at 30 ° C. for 3 to 5 days. The pH of the medium is 7.3. The composition of the medium is 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride, and 15 g of agar. SKS-6 bacteria can be stored by a freeze-drying method (stored at around + 5 ° C.), and in this case, the composition of the protective agent is 11
It is a 10% skim milk aqueous solution without pH adjustment sterilized at 0 ° C. for 10 minutes. To restore this, use sterile water at room temperature.

[0015] SKS-7 bacteria are facultative anaerobic bacteria belonging to intestinal bacteria. This bacterium was used on a tryptic soy agar medium (Eiken Chemical Co., Ltd., EM
C11) can be produced by culturing at 30 ° C. for 1 to 2 days.
The pH of the medium is 7.3. Further, the composition of the medium,
It is composed of 15 g of tryptone, 5 g of soypeptone, 5 g of sodium chloride and 15 g of agar. SKS-7
The bacterium can be stored by a freeze-drying method (stored at around + 5 ° C.), in which case the composition of the protective agent is a 10% non-PH adjusted skim milk aqueous solution sterilized at 110 ° C. for 10 minutes. To restore this, use sterile water at room temperature.

[0016] SKS-8 bacteria are aerobic bacteria belonging to fungi (molds). This bacterium can be produced by culturing on a potato dextrose agar medium (Eiken Chemical Co., E-MF06) sterilized at 121 ° C. for 15 minutes at 25 ° C. for 5 to 7 days. The pH of the medium is 5.6. The composition of the medium was 200 g of potato leachate and 20 g of glucose.
g, 15 g of agar. The SKS-8 bacteria can be stored by a freeze-drying method (stored at around + 5 ° C.), and the composition of the protective agent in this case is P sterilized at 110 ° C. for 10 minutes.
H-free 10% skim milk aqueous solution. To restore this, use sterile water at room temperature.

The SKS-9 bacterium is a knitted anaerobic bacterium belonging to the knitted anaerobic spore bacillus. This bacterium is 1
GAM agar medium "Nissui" sterilized under the condition of 5 minutes
(Nissui Pharmaceutical, 05421) at 35 ° C. for 2-3 days. The pH of the medium is 7.3. The cultivation of this bacterium is preferably anaerobic cultivation using a BBL Gaspak jar. The composition of the medium was 10 g of peptone, 3 g of soy peptone, 10 g of proteose peptone, 13.5 g of digested serum powder, 2.2 g of yeast extract, and 2.2 of meat extract.
g, liver extract 1.2 g, glucose 3 g, KH2 P04
2.5 g, Nacl 3 g, soluble starch 5 g, L-cysteine hydrochloride 0.3 g, sodium thioglycolate 0.1 g
3 g and 15 g of agar. SKS-9
The bacterium can be stored by a freeze-drying method (stored at around + 5 ° C.), in which case the composition of the protective agent is a 10% non-PH adjusted skim milk aqueous solution sterilized at 110 ° C. for 10 minutes. To restore this, use sterile water at room temperature.

The microbial cells SK can be obtained by mixing the SKS-1 to SKS-9 bacteria cultured as described above.
S is obtained. The mixing ratio may be the same ratio in weight ratio, but is not necessarily limited thereto, and the mixing ratio may be different. When mixed at the same ratio, the approximate number of bacteria per gram is as follows. SK
The S-1 strain is 4 × 10 8, and the SKS-2 strain is 1 × 10
8 ×, SKS-3 bacteria are 3 × 10 8 ×, SKS-
4 bacteria are 3 × 10 8, and SKS-5 bacteria are 2 × 10 8
2 × 10 7 for SKS-6 bacteria, 2 × 10 6 for SKS-7 bacteria, 1 × 10 6 for SKS-8 bacteria, 2 × 10 for SKS-9 bacteria Is the square of.

The above is an example of the culture of the SKS-1 to SKS-9 bacteria, which may be carried out by means other than those described above, and which are also included in the scope of the present invention. It is.

It has been experimentally found that such a microbial cell SKS activates useful microorganisms therein when mixed with other microbial cells. As a useful use for agriculture, when added to compost, it promotes the proliferation of useful fungi and bacteria, accelerates ripening, and decomposes well into fibrous material to become a useful compost and promotes soil activation. When added directly to the soil, vigorous propagation of soil bacteria and other bacteria is obtained, and the growth of the plant is accelerated. This is because microbial cells SKS are added to organic acids, amino acids and carbohydrates secreted from plants.
It is thought that microbial cells SKS excrete antibiotics, growth hormones, organic substances, and the like as metabolites thereof, and serve as nutrients for useful microorganisms to propagate.
In addition, it is expected that the crops will not be continuously cropped, that weeding will be effective, and that they will be used for planting trees.

The microbial cells SKS can be effectively used to decompose harmful substances, putrefactive substances and chemical substances to prevent pollution. The results of the experiment show that the microbial cells SKS are propagated throughout the raw sludge and sulfided by an extremely simple method of mixing and aging the microbial cells SKS with the marine sludge that emits a bad smell and is in trouble at the dump site. Remove harmful substances such as objects to make them harmless,
When the microbial cells SKS grown on the whole raw sludge are used as a soil activator, they further proliferate in the sprayed soil, promote the activation of soil microorganisms, and make a normal soil. Organic farming can be performed.
In addition, by adding to compost, propagation of useful bacteria contained in compost can be promoted and extremely useful compost can be produced in the same manner as described above. In addition, when marine sludge aged by adding microbial cells SKS is used as pottery clay, pottery with higher quality than conventional products can be obtained. Other uses related to pollution include sewage treatment and industrial wastewater treatment.

Furthermore, as for the production of industrial products, pure silicones can be obtained biologically by activating organosilicon bacteria,
By activating iron bacteria, it is possible to biologically obtain iron with uniform particles. For example, it can be used for organic matter decomposition of ore and labor saving of ore sorting. Furthermore, it can be used for the field of civil engineering such as chitin decomposition, soil drainage and water retention.

In addition, various applications are expected in the fields of food processing and medical and medical fields.

[0024]

As described above, the production method of the present invention
The microbial cell SKS obtained by the method has the property of activating the microorganism when it is mixed with various useful microbial cells. Make it comfortable and
It can be effectively used for industrial production, and not only can it increase production efficiency, but also can be expected to open up new technology fields that were not possible before.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI C12R 1:01) C12R 1:01)

Claims (1)

(57) [Claims] 1. A SKS-1 strain FERM BP-3692 belonging to Gram-positive spore-forming bacterium (mesophilic), and an SKS-2 strain FERM belonging to Gram-positive spore-forming bacterium (thermophilic).
BP-3693 , SKS-3 bacteria FERM BP-3694 belonging to catalase-positive gram-positive spore-free bacilli, SKS-4 bacteria FERM BP- belonging to actinomycetes (thermophilic)
3695 , SKS-5 bacteria FE belonging to actinomycetes (mesophilic)
RMBP-3696 , SKS-6 strain FERM BP-3697 belonging to catalase-positive Gram-positive cocci, SKS-7 strain FERM BP-3698 belonging to intestinal bacteria,
SKS-8 bacteria FERM BP-3 belonging to fungi (molds)
699 and SKS-9 belonging to knitting anaerobic spore-forming bacilli
A method for producing a microbial cell SKS, which comprises separately culturing the fungi FERM BP-3700 , and then mixing and culturing the cultures.