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US20080182324A1 - Method and reagent system with non-regenerable enzyme-coenzyme complex - Google Patents

Method and reagent system with non-regenerable enzyme-coenzyme complex Download PDF

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US20080182324A1
US20080182324A1 US12/008,283 US828308A US2008182324A1 US 20080182324 A1 US20080182324 A1 US 20080182324A1 US 828308 A US828308 A US 828308A US 2008182324 A1 US2008182324 A1 US 2008182324A1
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coenzyme
enzyme
reagent system
reagent
analyte
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US7951581B2 (en
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Carina Horn
Joachim Hoenes
Wolfgang-Reinhold Knappe
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Roche Diabetes Care Inc
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Roche Diagnostics Operations Inc
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Priority claimed from DE2002121846 external-priority patent/DE10221846A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Definitions

  • the invention relates to a method and a reagent system for detecting an analyte in a sample through an enzymatic reaction, comprising the use of an enzyme-coenzyme complex as non-regenerable, in particular stoichiometric reactant for the analyte present in the sample.
  • analytes for example glucose in blood
  • enzymatic methods entail the analyte to be determined being brought into contact with a suitable enzyme and a coenzyme, the enzyme being employed in catalytic amounts.
  • the redox equivalents produced on reduction or oxidation of the coenzyme are transferred to mediators which are then detected electrochemically or photometrically in a further step.
  • a calibration provides a direct connection between the measurement and the concentration of the analyte to be determined.
  • Narayanaswamy et al. (Analytical Letters 21 (7) (1988), 1165-1175) describe a fluorescence measurement with glucose dehydrogenase and NAD for glucose determination.
  • the enzyme is in this case employed in catalytic, i.e. non-stoichiometric, amounts.
  • the fluorescence measurement detects the free NADH in the solution.
  • the mediators often also have to be employed in large excess relative to the enzyme-coenzyme system.
  • the coenzyme has a high reactivity, so that the enzymic activity declines markedly on decomposition of the mediator, even in small amounts, e.g. ⁇ 1% or on exposure to foreign substances, e.g. volatilization of the substances from packaging materials. This may lead to false signals in the determination of the analyte.
  • the determination times for the analytes are normally in the region of at least a few seconds, for example for glucose in the region of >4 s, and the required sample volumes are large, e.g. >0.5 ⁇ l.
  • the object on which the present invention was based is at least partly to avoid the described disadvantages of the prior art. It was particularly intended to provide a non-sensitive and rapid method for the enzymatic detection of analytes, which leads to reliable measurement results even in the absence of mediators or/and indicators.
  • This object is achieved by using an enzyme-coenzyme complex as stoichiometric reactant instead of, as usual, as catalyst. Detection of the analyte requires only a single reaction step and is therefore extremely fast. The use of mediators and indicators, associated with the employment of complex reaction mixtures, with low stability and high susceptibility to interference, is no longer necessary.
  • the present invention provides one aspect of the invention is thus a method for detecting an analyte in a sample by an enzymatic reaction, comprising the steps:
  • a further aspect of the invention is a reagent system for detecting an analyte in a sample, comprising:
  • a detection reagent comprising an enzyme-coenzyme complex, where no regeneration of the coenzyme takes place
  • the present invention makes a simple qualitative or quantitative determination of analytes possible within a very short reaction time of, preferably, ⁇ 5 s, particularly preferably ⁇ 1 s, most preferably ⁇ 0.1 s.
  • the reaction is carried out under conditions with which no regeneration of the coenzyme takes place during the determination. It is moreover possible for a molecule enzyme-coenzyme complex to react only with a single molecule of the analyte.
  • the reaction is therefore expediently carried out in the absence of mediators or other substances able to bring about regeneration of the coenzyme.
  • the detection reagent comprises the enzyme-coenzyme complex in an amount sufficient to make qualitative or/and quantitative determination of the analyte possible according to the desired test format.
  • the enzyme-coenzyme complex is employed in an amount such that the number of reacting molecules of the enzyme-coenzyme complex correlates with the analyte concentration present in the sample.
  • the enzyme-coenzyme complex is particularly preferably employed in an at least stoichiometric amount relative to the analyte present in the sample, preferably in a stoichiometric excess relative to the analyte.
  • the statement “in at least a stoichiometric amount” means that the size of the sample is adjusted relative to the number of molecules of the enzyme-coenzyme complex in such a way that, with the analyte concentrations to be expected in the sample, the number of molecules of the enzyme-coenzyme complex which react with the analyte correlates with the analyte concentration present in the sample.
  • “Stoichiometric amount” preferably means that the number of molecules of the enzyme-coenzyme complex corresponds to the maximum number of analyte molecules to be expected in the investigative sample.
  • the method and the detection system permit the use of very small amounts of sample, for example sample volumes of ⁇ 1 ⁇ l, in particular ⁇ 0.1 ⁇ l.
  • sample can where appropriate also be diluted before contacting with the detection reagent.
  • the method and detection system of the invention is suitable for determining any analytes, for example parameters in body fluids such as, for example, blood, serum, plasma or urine, but also in effluent samples or foodstuffs.
  • the method can also be carried out as wet test, e.g. in a cuvette, or as dry test on an appropriate reagent support.
  • the analytes which can be determined are any biological or chemical substances which are able to undergo a reaction, in particular a redox reaction, with an enzyme-coenzyme complex, such as, for example, glucose, lactic acid, malic acid, glycerol, alcohol, cholesterol, triglycerides, ascorbic acid, cysteine, glutathione, peptides etc.
  • an enzyme-coenzyme complex such as, for example, glucose, lactic acid, malic acid, glycerol, alcohol, cholesterol, triglycerides, ascorbic acid, cysteine, glutathione, peptides etc.
  • the enzymatic reaction is preferably a redox reaction in which the coenzyme in the enzyme-coenzyme complex is reduced or oxidized.
  • the enzyme preferably used for a reaction of this type is an oxidoreductase.
  • the enzyme particularly preferably used is a dehydrogenase, for example selected from a glucose dehydrogenase (E.C. 1.1.1.47), lactate dehydrogenase (E.C. 1.1.1.27, 1.1.1.28), malate dehydrogenase (E.C. 1.1.1.37), glycerol dehydrogenase (E.C. 1.1.1.6), alcohol dehydrogenase (E.C.
  • oxidases such as, for example, glucose oxidase (E.C.1.1.3.4) or cholesterol oxidase (E.C.1.1.3.6).
  • Coenzymes for the purposes of the present invention are preferably organic molecules which are linked covalently or noncovalently to an enzyme and are changed, for example oxidized or reduced, by the conversion of the analyte.
  • Preferred examples of coenzymes are flavin, nicotine and quinone derivatives, for example flavin nucleoside derivatives such as, for example, FAD, FADH 2 , FMN, FMNH 2 , etc., nicotine nucleoside derivatives such as, for example, NAD + , NADH/H + , NADP + , NADPH/H + etc. or ubiquinones such as, for example, coenzyme Q, PQQ etc.
  • the change in the coenzyme through reaction with the analyte can in principle be detected in any manner. It is possible in principle to employ for this all methods known in the art for detecting enzymatic reactions.
  • the change in the coenzyme is preferably detected by optical methods. Optical detection methods include for example measuring absorption, fluorescence, circular dichroism (CD), optical rotary dispersion (ORD), refractometry etc.
  • the change in the coenzyme is particularly preferably detected by measuring the fluorescence. The fluorescence measurement is highly sensitive and makes it possible to detect even low concentrations of the analyte in miniaturized systems.
  • the method or detection system of the invention may comprise a liquid test, in which case the reagent is present for example in the form of a solution or suspension in an aqueous or nonaqueous liquid or as powder or lyophilizate.
  • the method and detection system of the invention preferably comprises a dry test, in which case the reagent is applied to a support.
  • the support may comprise for example a test strip comprising an absorbent or/and swellable material which is wetted by the sample liquid to be investigated.
  • the detection reagent used is a gel matrix with an enzyme-coenzyme complex embedded therein.
  • the gel matrix preferably has a layer thickness of ⁇ 50 ⁇ m, in particular ⁇ 5 ⁇ m, and is applied to a support, for example an at least partly optically transparent support.
  • the gel matrix may be a matrix comprising one or more soluble polymers, as in known dry test systems (e.g. AccuChek Active), and can be produced by knife application and drying.
  • the matrix is preferably a polymer with a structure based on photopolymerizable substances such as, for example, acrylic monomers, e.g.
  • a gel matrix of this type can be produced by applying a liquid which contains the reagent, comprising enzyme, photopolymerizable monomer and, where appropriate, coenzyme, photoinitiator or/and unreactive constituents, to an at least partly optically transparent support, for example to a plastics sheet, and irradiating, for example with UV light from the reverse side, so that polymerization of the monomer or of the monomers takes place on the support up to a predefined layer thickness.
  • the layer thickness can be controlled by adding absorbing substances to the reagent or/and through the duration or intensity of irradiation. Excess liquid reagent can be removed and reused after the polymerization (see, for example, FIG. 2 ).
  • the gel matrix can also be produced by conventional coating procedures, in which case the liquid reagent is applied to a support, brought to the desired thickness using suitable methods, e.g. using a knife, and then completely polymerized.
  • the enzyme After inclusion by polymerization or embedding in the gel matrix, the enzyme is in a protected microenvironment. If the polymeric gel matrix is sufficiently crosslinked, the enzyme molecules are present in an immobilized form. Low molecular weight substances or glucose or other analytes or else coenzymes can, however, diffuse freely through the polymer network.
  • the enzyme can either be included together with its coenzyme by polymerization in the matrix or, after the polymerization, the matrix can be brought into contact with a solution of the coenzyme, so that the appropriate enzyme-coenzyme complex is formed.
  • concentration of the enzyme in the gel matrix is preferably chosen to be high enough for a stoichiometric reaction with the analyte to be determined, and a direct determination of the coenzyme which is changed by the reaction, to be possible.
  • the reaction consists only of a single catalytic reaction, for example, a redox reaction, which can take place in the region of milliseconds or microseconds.
  • the coenzyme which is changed by the reaction is optimally protected from interfering influences through binding to the active center of the enzyme and, where appropriate, additionally by embedding in the gel matrix.
  • FIG. 1 shows a first embodiment of the detection system of the invention.
  • a reagent layer ( 2 ) e.g. a gel matrix with an enzyme-coenzyme complex
  • the enzyme-coenzyme complex is in a form such that no regeneration of the coenzyme can take place during the analyte determination.
  • a sample ( 3 ) e.g. blood, is put on the reagent layer. Determination of the enzymatic reaction between the analyte contained in the sample ( 3 ), and the enzyme-coenzyme complex contained in the reagent layer ( 2 ) takes place by optical methods.
  • a laser or an LED is beamed from behind (through the support) onto the reagent layer ( 2 ).
  • Absorption light or fluorescent light beamed back from the sample is detected in a detector ( 5 ).
  • an optical filter element ( 6 ) is put in front of the detector in order to block leakage of the fluorescence-exciting light.
  • FIG. 2 shows the production of a detection system of the invention.
  • a liquid reagent ( 12 ) is applied, for example in a first position ( 13 ), to an optically transparent support ( 11 ), e.g. a plastics sheet.
  • the liquid reagent ( 12 ) is irradiated at a second position from below through the support ( 11 ) with light from a light source ( 14 ).
  • the support is moved in the direction ( 15 ) identified by the arrow.
  • a polymerized reagent layer ( 16 ) is formed directly on the support ( 11 ). Excess liquid reagent is present above the polymer layer ( 16 ).
  • the thickness of the polymerized reagent layer ( 16 ) can be controlled through the reagent composition, the duration and intensity of the beaming in of light, and through the properties of the support ( 11 ).
  • FIG. 3 shows an embodiment of a fluorescence-based sensor from below.
  • a polymerized reagent layer for example one produced by the continuous process in FIG. 2 , can be cut and applied to a support ( 21 ) by use of known techniques.
  • exciting light e.g. UV light
  • the fluorescence 24 ), e.g. blue light, generated through the reaction of the analyte with the enzyme-coenzyme complex in the reagent layer ( 22 ) is detected with a detector.
  • a plurality of (identical or different) reagents can be applied to a support.
  • One example of such an embodiment in the form of a disk is shown in FIG. 4 .
  • a plurality of reagent spots ( 62 ) is disposed on the optically transparent support ( 61 ).
  • FIGS. 5A and 5B show the fluorescence of a detection system of the invention (glucose dehydrogenase and NAD + ) with increasing glucose concentration under a CCD camera.
  • the term “substantially” is utilized herein to represent the inherent degree of uncertainty that may be attributed to any quantitative comparison, value, measurement, or other representation.
  • the term “substantially” is also utilized herein to represent the degree by which a quantitative representation may very from a stated reference without resulting in a change in the basic function of the subject matter at issue.
  • the solution with the enzyme system does not fluoresce without glucose. Nor do glucose and NAD + result in any fluorescence.
  • Amount Weight Substance [g] [%] Acrylamide 2.5 22.02 Methylenebisacrylamide 0.7 6.17 2,2-Dimethoxy-2-phenylacetophenone 0.05 0.44 Glycerol 5 44.05 Hydroxyethyl methacrylate 1.4 12.33 Methyl methacrylate 0.4 3.52 Crodasinic O solution, pH 8, 0.3 g/1000 ml 1 8.81 N,N′-(1,2-Dihydroxyethylene)bisacrylamide 0.3 2.64 TOTAL 11.35 100
  • the clear solution was poured onto a corona-treated polycarbonate sheet 125 mm thick and illuminated with a conventional illumination apparatus (Isel UV illumination device 2 ) for 20 min.
  • the sheet was briefly washed with water and then dried in the air.
  • the resulting layer thickness was ⁇ 2 ⁇ m.
  • a freshly prepared glucose/NAD + solution (GKL-3 solution, 300 mg/dl glucose, 1 ml/6.4 mg of NAD + ) was spotted on the film. A strong fluorescence was immediately visible under a UV lamp.
  • a polymer layer comprising a blue dye (absorption maximum ⁇ 650 nm) for better identification was produced (formula 2).
  • a yellow dye was admixed as UV absorber to the initial formula (formula 3).
  • the mixture was homogenized by stirring and by ultrasonic bath treatment, distributed with a pipette on a 140 ⁇ m Pokalon sheet (corona-treated, stage 4) and illuminated in a UV illumination device (Actina U4, W. Lemmen GmbH) for 1 min.
  • the resulting layer thickness was measured with a screw gage and was 240.5 ⁇ m.
  • Substance Amount Weight [%] Formula 2 1 ml ca. 99.99 Mordant Yellow 7 (No. 686) 0.0001 g 0.001 (UV absorber) TOTAL ca. 1.0001 g 100
  • the mixture was distributed on a sheet and then polymerized as described above.
  • the resulting layer thickness was measured with a screw gage and was 79.3 ⁇ m.

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Abstract

The present invention is generally directed towards a reagent system for detecting an analyte in a sample. The reagent system has a detection reagent comprising an enzyme-coenzyme complex in a form such that no regeneration of the coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and a support to receive the detection reagent.

Description

    TECHNICAL FIELD
  • The invention relates to a method and a reagent system for detecting an analyte in a sample through an enzymatic reaction, comprising the use of an enzyme-coenzyme complex as non-regenerable, in particular stoichiometric reactant for the analyte present in the sample.
  • The detection of analytes, for example glucose in blood, by enzymatic methods is known. These entail the analyte to be determined being brought into contact with a suitable enzyme and a coenzyme, the enzyme being employed in catalytic amounts. The redox equivalents produced on reduction or oxidation of the coenzyme are transferred to mediators which are then detected electrochemically or photometrically in a further step. A calibration provides a direct connection between the measurement and the concentration of the analyte to be determined.
    • BACKGROUND
  • Sierra et al. (Anal. Chem. 69 (1997), 1471-1476) describe a determination of blood glucose based on the intrinsic fluorescence of glucose oxidase. In this method too, the enzyme is employed together with its coenzyme FAD in catalytic amounts, with redox equivalents being transferred to oxygen as mediator.
  • Narayanaswamy et al. (Analytical Letters 21 (7) (1988), 1165-1175) describe a fluorescence measurement with glucose dehydrogenase and NAD for glucose determination. The enzyme is in this case employed in catalytic, i.e. non-stoichiometric, amounts. The fluorescence measurement detects the free NADH in the solution.
  • It is possible through the electrochemically active substances (mediators) required for the prior art detection systems to detect the analytes to be determined only indirectly, i.e. via a plurality of chemical reactions. For this purpose, a complicated adjustment of the concentrations of the substances involved to optimize the reaction rate is often necessary. There is moreover the risk that the required electrochemically active substances are unstable on prolonged storage.
  • The mediators often also have to be employed in large excess relative to the enzyme-coenzyme system. The coenzyme has a high reactivity, so that the enzymic activity declines markedly on decomposition of the mediator, even in small amounts, e.g. <1% or on exposure to foreign substances, e.g. volatilization of the substances from packaging materials. This may lead to false signals in the determination of the analyte. Yet a further disadvantage is that the determination times for the analytes are normally in the region of at least a few seconds, for example for glucose in the region of >4 s, and the required sample volumes are large, e.g. >0.5 μl.
  • The object on which the present invention was based is at least partly to avoid the described disadvantages of the prior art. It was particularly intended to provide a non-sensitive and rapid method for the enzymatic detection of analytes, which leads to reliable measurement results even in the absence of mediators or/and indicators.
  • This object is achieved by using an enzyme-coenzyme complex as stoichiometric reactant instead of, as usual, as catalyst. Detection of the analyte requires only a single reaction step and is therefore extremely fast. The use of mediators and indicators, associated with the employment of complex reaction mixtures, with low stability and high susceptibility to interference, is no longer necessary.
    • SUMMARY
  • It is against the above background that the present invention proves certain unobvious advantages and advancements over the prior art. In particular, the inventor has recognized a need for improvements in a method and reagent system with non- regenerable enzyme-coenzyme complex.
  • Although the present invention is not limited to specific advantage or functionality, it is noted that the present invention provides one aspect of the invention is thus a method for detecting an analyte in a sample by an enzymatic reaction, comprising the steps:
  • contacting the sample with a detection reagent comprising an enzyme-coenzyme complex, where no regeneration of the coenzyme takes place, and
  • detecting a reaction of the analyte through a change in the enzyme-coenzyme complex.
  • A further aspect of the invention is a reagent system for detecting an analyte in a sample, comprising:
  • a detection reagent comprising an enzyme-coenzyme complex, where no regeneration of the coenzyme takes place, and
      • a support to receive the detection reagent.
  • The present invention makes a simple qualitative or quantitative determination of analytes possible within a very short reaction time of, preferably, <5 s, particularly preferably ≦1 s, most preferably ≦0.1 s. The reaction is carried out under conditions with which no regeneration of the coenzyme takes place during the determination. It is moreover possible for a molecule enzyme-coenzyme complex to react only with a single molecule of the analyte. The reaction is therefore expediently carried out in the absence of mediators or other substances able to bring about regeneration of the coenzyme.
  • The detection reagent comprises the enzyme-coenzyme complex in an amount sufficient to make qualitative or/and quantitative determination of the analyte possible according to the desired test format. In particular, for quantitative determination of the analyte, the enzyme-coenzyme complex is employed in an amount such that the number of reacting molecules of the enzyme-coenzyme complex correlates with the analyte concentration present in the sample. The enzyme-coenzyme complex is particularly preferably employed in an at least stoichiometric amount relative to the analyte present in the sample, preferably in a stoichiometric excess relative to the analyte. In this connection, the statement “in at least a stoichiometric amount” means that the size of the sample is adjusted relative to the number of molecules of the enzyme-coenzyme complex in such a way that, with the analyte concentrations to be expected in the sample, the number of molecules of the enzyme-coenzyme complex which react with the analyte correlates with the analyte concentration present in the sample. “Stoichiometric amount” preferably means that the number of molecules of the enzyme-coenzyme complex corresponds to the maximum number of analyte molecules to be expected in the investigative sample.
  • The method and the detection system permit the use of very small amounts of sample, for example sample volumes of ≦1 μl, in particular ≦0.1 μl. The sample can where appropriate also be diluted before contacting with the detection reagent.
  • The method and detection system of the invention is suitable for determining any analytes, for example parameters in body fluids such as, for example, blood, serum, plasma or urine, but also in effluent samples or foodstuffs. The method can also be carried out as wet test, e.g. in a cuvette, or as dry test on an appropriate reagent support.
  • The analytes which can be determined are any biological or chemical substances which are able to undergo a reaction, in particular a redox reaction, with an enzyme-coenzyme complex, such as, for example, glucose, lactic acid, malic acid, glycerol, alcohol, cholesterol, triglycerides, ascorbic acid, cysteine, glutathione, peptides etc.
  • The enzymatic reaction is preferably a redox reaction in which the coenzyme in the enzyme-coenzyme complex is reduced or oxidized. The enzyme preferably used for a reaction of this type is an oxidoreductase. The enzyme particularly preferably used is a dehydrogenase, for example selected from a glucose dehydrogenase (E.C. 1.1.1.47), lactate dehydrogenase (E.C. 1.1.1.27, 1.1.1.28), malate dehydrogenase (E.C. 1.1.1.37), glycerol dehydrogenase (E.C. 1.1.1.6), alcohol dehydrogenase (E.C. 1.1.1.1) or amino-acid dehydrogenase, e.g. L-amino-acid dehydrogenase (E.C.1.4.1.5). Further suitable enzymes are oxidases such as, for example, glucose oxidase (E.C.1.1.3.4) or cholesterol oxidase (E.C.1.1.3.6).
  • Coenzymes for the purposes of the present invention are preferably organic molecules which are linked covalently or noncovalently to an enzyme and are changed, for example oxidized or reduced, by the conversion of the analyte. Preferred examples of coenzymes are flavin, nicotine and quinone derivatives, for example flavin nucleoside derivatives such as, for example, FAD, FADH2, FMN, FMNH2, etc., nicotine nucleoside derivatives such as, for example, NAD+, NADH/H+, NADP+, NADPH/H+ etc. or ubiquinones such as, for example, coenzyme Q, PQQ etc.
  • The change in the coenzyme through reaction with the analyte can in principle be detected in any manner. It is possible in principle to employ for this all methods known in the art for detecting enzymatic reactions. However, the change in the coenzyme is preferably detected by optical methods. Optical detection methods include for example measuring absorption, fluorescence, circular dichroism (CD), optical rotary dispersion (ORD), refractometry etc. The change in the coenzyme is particularly preferably detected by measuring the fluorescence. The fluorescence measurement is highly sensitive and makes it possible to detect even low concentrations of the analyte in miniaturized systems.
  • The method or detection system of the invention may comprise a liquid test, in which case the reagent is present for example in the form of a solution or suspension in an aqueous or nonaqueous liquid or as powder or lyophilizate. However, the method and detection system of the invention preferably comprises a dry test, in which case the reagent is applied to a support. The support may comprise for example a test strip comprising an absorbent or/and swellable material which is wetted by the sample liquid to be investigated.
  • In a particularly preferred embodiment, the detection reagent used is a gel matrix with an enzyme-coenzyme complex embedded therein. The gel matrix preferably has a layer thickness of ≦50 μm, in particular ≦5 μm, and is applied to a support, for example an at least partly optically transparent support. The gel matrix may be a matrix comprising one or more soluble polymers, as in known dry test systems (e.g. AccuChek Active), and can be produced by knife application and drying. The matrix is preferably a polymer with a structure based on photopolymerizable substances such as, for example, acrylic monomers, e.g. acrylamide or/and acrylic esters such as polyethylene glycol diacrylate, or vinylaromatic monomers, e.g. 4-vinylbenzenesulfonic acid, or combinations thereof. A gel matrix of this type can be produced by applying a liquid which contains the reagent, comprising enzyme, photopolymerizable monomer and, where appropriate, coenzyme, photoinitiator or/and unreactive constituents, to an at least partly optically transparent support, for example to a plastics sheet, and irradiating, for example with UV light from the reverse side, so that polymerization of the monomer or of the monomers takes place on the support up to a predefined layer thickness. The layer thickness can be controlled by adding absorbing substances to the reagent or/and through the duration or intensity of irradiation. Excess liquid reagent can be removed and reused after the polymerization (see, for example, FIG. 2).
  • On the other hand, the gel matrix can also be produced by conventional coating procedures, in which case the liquid reagent is applied to a support, brought to the desired thickness using suitable methods, e.g. using a knife, and then completely polymerized.
  • After inclusion by polymerization or embedding in the gel matrix, the enzyme is in a protected microenvironment. If the polymeric gel matrix is sufficiently crosslinked, the enzyme molecules are present in an immobilized form. Low molecular weight substances or glucose or other analytes or else coenzymes can, however, diffuse freely through the polymer network.
  • The enzyme can either be included together with its coenzyme by polymerization in the matrix or, after the polymerization, the matrix can be brought into contact with a solution of the coenzyme, so that the appropriate enzyme-coenzyme complex is formed. The concentration of the enzyme in the gel matrix is preferably chosen to be high enough for a stoichiometric reaction with the analyte to be determined, and a direct determination of the coenzyme which is changed by the reaction, to be possible. The reaction consists only of a single catalytic reaction, for example, a redox reaction, which can take place in the region of milliseconds or microseconds. The coenzyme which is changed by the reaction is optimally protected from interfering influences through binding to the active center of the enzyme and, where appropriate, additionally by embedding in the gel matrix.
  • The invention is additionally to be explained by the following figures and examples.
  • These and other features and advantages of the present invention will be more fully understood from the following detailed description of the invention taken together with the accompanying claims. It is noted that the scope of the claims is definitely by the recitations therein and not by the specific discussion of the features and advantages set forth in the present description.
    • BRIEF DESCRIPTION OF THE DRAWINGS
  • The following detailed description of the embodiments of the present invention can be best understood when read in conjunction with the following drawings, where like structure is indicated with like reference numerals and in which:
  • FIG. 1 shows a first embodiment of the detection system of the invention. A reagent layer (2), e.g. a gel matrix with an enzyme-coenzyme complex, is applied to an optically transparent support (1). The enzyme-coenzyme complex is in a form such that no regeneration of the coenzyme can take place during the analyte determination. A sample (3), e.g. blood, is put on the reagent layer. Determination of the enzymatic reaction between the analyte contained in the sample (3), and the enzyme-coenzyme complex contained in the reagent layer (2) takes place by optical methods. Light from a light source (4), e.g. a laser or an LED, is beamed from behind (through the support) onto the reagent layer (2). Absorption light or fluorescent light beamed back from the sample is detected in a detector (5). Where appropriate—in particular for detecting fluorescent light—an optical filter element (6) is put in front of the detector in order to block leakage of the fluorescence-exciting light.
  • FIG. 2 shows the production of a detection system of the invention. A liquid reagent (12) is applied, for example in a first position (13), to an optically transparent support (11), e.g. a plastics sheet. The liquid reagent (12) is irradiated at a second position from below through the support (11) with light from a light source (14). At the same time, the support is moved in the direction (15) identified by the arrow. A polymerized reagent layer (16) is formed directly on the support (11). Excess liquid reagent is present above the polymer layer (16). The thickness of the polymerized reagent layer (16) can be controlled through the reagent composition, the duration and intensity of the beaming in of light, and through the properties of the support (11).
  • FIG. 3 shows an embodiment of a fluorescence-based sensor from below. A polymerized reagent layer, for example one produced by the continuous process in FIG. 2, can be cut and applied to a support (21) by use of known techniques. After application of the sample to the upper side, exciting light (23), e.g. UV light, is beamed in from a light source from below. The fluorescence (24), e.g. blue light, generated through the reaction of the analyte with the enzyme-coenzyme complex in the reagent layer (22) is detected with a detector.
  • It is also possible for a plurality of (identical or different) reagents to be applied to a support. One example of such an embodiment in the form of a disk is shown in FIG. 4. A plurality of reagent spots (62) is disposed on the optically transparent support (61).
  • FIGS. 5A and 5B show the fluorescence of a detection system of the invention (glucose dehydrogenase and NAD+) with increasing glucose concentration under a CCD camera.
    •
  • Skilled artisans appreciate that elements in the figures are illustrated for simplicity and clarity and have not necessarily been drawn to scale. For example, the dimensions of some of the elements in the figure may be exaggerated relative to other elements to help improve understanding of the embodiment(s) of the present invention.
  • In order that the invention may be more readily understood, reference is made to the following examples, which are intended to illustrate the invention, but not limit the scope thereof.
    • DETAILED DESCRIPTION
  • The following description of the preferred embodiment is merely exemplary in nature and is in no way intended to limit the invention or its application or uses.
  • It is noted that terms like “preferably”, “commonly”, and “typically” are not utilized herein to limit the scope of the claimed invention or to imply that certain features are critical, essential, or even important to the structure or function of the claimed invention. Rather, these terms are merely intended to highlight alternative or additional features that may or may not be utilized in a particular embodiment of the present invention.
  • For the purposes of describing and defining the present invention it is noted that the term “substantially” is utilized herein to represent the inherent degree of uncertainty that may be attributed to any quantitative comparison, value, measurement, or other representation. The term “substantially” is also utilized herein to represent the degree by which a quantitative representation may very from a stated reference without resulting in a change in the basic function of the subject matter at issue.
    • EXAMPLE 1 Stoichiometric Detection of Glucose in the Glucose Dehydrogenase (GlucDH)/NAD+ System in a Cuvette
  • 100 mg/ml GlucDH are dissolved in buffer of pH 7 and mixed with the appropriate amount of NAD+. On addition of increasing amounts of glucose, an increase in the fluorescence can be detected visually under a UV lamp (excitation wavelength 366 nm) (FIGS. 5A and 5B).
  • The solution with the enzyme system does not fluoresce without glucose. Nor do glucose and NAD+ result in any fluorescence.
    • EXAMPLE 2 Detection of Glucose in the GlucDH/NAD+ System in a Polymer Film
  • A suspension of the following substance was mixed in a plastic test tube
    • Formula 1
  • Amount Weight
    Substance [g] [%]
    Acrylamide 2.5 22.02
    Methylenebisacrylamide 0.7 6.17
    2,2-Dimethoxy-2-phenylacetophenone 0.05 0.44
    Glycerol 5 44.05
    Hydroxyethyl methacrylate 1.4 12.33
    Methyl methacrylate 0.4 3.52
    Crodasinic O solution, pH 8, 0.3 g/1000 ml 1 8.81
    N,N′-(1,2-Dihydroxyethylene)bisacrylamide 0.3 2.64
    TOTAL 11.35 100
  • 0.5 ml of this suspension were mixed with 0.5 ml of a solution of GlucDH (100 mg/ml), and the mixture was homogenized free of air bubbles in an ultrasonic bath.
  • The clear solution was poured onto a corona-treated polycarbonate sheet 125 mm thick and illuminated with a conventional illumination apparatus (Isel UV illumination device 2) for 20 min. The sheet was briefly washed with water and then dried in the air.
  • The resulting layer thickness was <2 μm. A freshly prepared glucose/NAD+ solution (GKL-3 solution, 300 mg/dl glucose, 1 ml/6.4 mg of NAD+) was spotted on the film. A strong fluorescence was immediately visible under a UV lamp.
    • EXAMPLE 3 Adding a UV Absorber to Influence the Layer Thickness
  • A polymer layer comprising a blue dye (absorption maximum≈650 nm) for better identification was produced (formula 2). In a further experiment, a yellow dye was admixed as UV absorber to the initial formula (formula 3).
    • Formula 2
  • Weight
    Substance Amount [%]
    Acrylamide  37.5 g (0.53 mol) 25.78
    Polyethylene glycol diacrylate,  52.5 g (ca. 0.96 mol) 36.10
    Mw ≈ 575 g/mol
    Solution of Crodasinic O (0.3 g/1 l)    50 g 34.38
    4-Vinylbenzenesulfonic acid    5 g 3.44
    2,2-Dimethoxy-2-   350 mg 0.24
    phenylacetophenone photoinitiator
    New methylene blue N   100 mg 0.06
    TOTAL 145.45 g 100
  • The mixture was homogenized by stirring and by ultrasonic bath treatment, distributed with a pipette on a 140 μm Pokalon sheet (corona-treated, stage 4) and illuminated in a UV illumination device (Actina U4, W. Lemmen GmbH) for 1 min. The resulting layer thickness was measured with a screw gage and was 240.5 μm.
    • Formula 3
  • Substance Amount Weight [%]
    Formula 2 1 ml ca. 99.99
    Mordant Yellow 7 (No. 686) 0.0001 g 0.001
    (UV absorber)
    TOTAL ca. 1.0001 g 100
  • The mixture was distributed on a sheet and then polymerized as described above. The resulting layer thickness was measured with a screw gage and was 79.3 μm.
  • This experiment shows that it is possible to influence the layer thickness. With reaction conditions which were otherwise the same, the layer thickness without UV absorber is 240.5 μm (see above); only 79.3 μm with UV absorber (Mordant Yellow 7).
  • Having described the invention in detail and by reference to specific embodiments thereof, it will be apparent that modification and variations are possible without departing from the scope of the invention defined in the appended claims. More specifically, although some aspects of the present invention are identified herein as preferred or particularly advantageous, it is contemplated that the present invention is not necessarily limed to these preferred aspects of the invention.

Claims (12)

1: A reagent system for detecting an analyte in a sample, comprising:
(a) a detection reagent comprising an enzyme-coenzyme complex in a form such that no regeneration of the coenzyme takes place, whereby the enzyme-coenzyme complex is employed in an at least stoichiometric amount relative to the analyte present in the sample, and
(b) a support to receive the detection reagent.
2: The reagent system as claimed in claim 1, characterized in that an oxidoreductase is used as the enzyme, and a change in the coenzyme due to oxidation or reduction is detected.
3: The reagent system as claimed in claim 1, characterized in that a coenzyme is selected from nicotine derivatives is used.
4: The reagent system as claimed in claim 1, characterized in that the change in the coenzyme is detected by optical methods.
5: The reagent system as claimed in claim 1, characterized in that the enzyme-coenzyme complex is embedded in a gel matrix.
6: The reagent system as claimed in claim 1, characterized in that the gel matrix has a layer thickness of ≦50 μm.
7: The reagent system as claimed in claim 1, characterized in that the support is at least partly optically transparent.
8: The reagent system as claimed in claim 1, characterized in that the support comprises an essentially planar structure.
9: The reagent system as claimed in claim 1, characterized in that the support comprises a plurality of different detection reagents.
10: The reagent system as claimed in claim 1, characterized in that an analyte in a body fluid is detected.
11: The reagent system as claimed in claim 1, characterized in that a determination of glucose in the blood is carried out.
12: The reagent system as claimed in claim 1, characterized in that the duration of the reaction of the analyte is ≦5 s.
US12/008,283 2002-05-16 2008-01-10 Method and reagent system with non-regenerable enzyme-coenzyme complex Expired - Fee Related US7951581B2 (en)

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DE10221840 2002-05-16
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DE2002121845 DE10221845A1 (en) 2002-05-16 2002-05-16 Detecting analyte by enzymatic reaction, useful specifically for measuring glucose in blood, based on reaction with enzyme-coenzyme complex
DE10221840.4 2002-05-16
DE10221512.5 2002-05-16
DE2002121846 DE10221846A1 (en) 2002-05-16 2002-05-16 Detecting an analyte by enzymatic reaction, useful specifically for measuring glucose in blood, based on reaction with coenzyme and inactive coenzyme-binding protein
DE10221845 2002-05-16
DE2002121840 DE10221840A1 (en) 2002-05-16 2002-05-16 Production of polymer layers on a transparent support, for use in sensors, e.g. for blood analysis, comprises coating the support with a photopolymerizable liquid composition and irradiating the liquid through the support
PCT/EP2003/005178 WO2003097864A1 (en) 2002-05-16 2003-05-16 Method and reagent system having a non-regenerative enzyme-coenzyme complex
US10/514,451 US7341830B2 (en) 2002-05-16 2003-05-16 Method and reagent system having a non-regenerative enzyme-coenzyme complex
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Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7341830B2 (en) * 2002-05-16 2008-03-11 Roche Diagnostics Operations, Inc. Method and reagent system having a non-regenerative enzyme-coenzyme complex
DE10325699B3 (en) * 2003-06-06 2005-02-10 Roche Diagnostics Gmbh System for analyzing a sample to be tested and using such a system
DE102004007983A1 (en) * 2004-02-18 2005-09-08 Roche Diagnostics Gmbh Test element with single-layer reaction film
DE102004051830B4 (en) * 2004-10-25 2007-12-13 Roche Diagnostics Gmbh Multifunctional reference system for analyte determination by fluorescence
US20060281187A1 (en) 2005-06-13 2006-12-14 Rosedale Medical, Inc. Analyte detection devices and methods with hematocrit/volume correction and feedback control
DE102005035461A1 (en) 2005-07-28 2007-02-15 Roche Diagnostics Gmbh Stable NAD / NADH derivatives
US8801631B2 (en) 2005-09-30 2014-08-12 Intuity Medical, Inc. Devices and methods for facilitating fluid transport
EP1928302B1 (en) 2005-09-30 2012-08-01 Intuity Medical, Inc. Fully integrated wearable or handheld monitor
CA2564666A1 (en) * 2005-10-25 2007-04-25 F. Hoffmann-La Roche Ag Fluorescence spectroscopy in absorbing media
GB0526051D0 (en) * 2005-12-21 2006-02-01 Oxford Biosensors Ltd Cholesterol sensor
EP1830177A1 (en) * 2006-03-02 2007-09-05 F. Hoffman-la Roche AG Integrated test element
WO2007147086A2 (en) * 2006-06-14 2007-12-21 Brigham Young University Adsorption-resistant acrylic copolymer for fluidic devices
EP2022859A1 (en) * 2007-08-01 2009-02-11 Roche Diagnostics GmbH Method and device for determining the concentration of an analyte using measurement of fluorescence
EP2093284A1 (en) 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisation of dehydrogenases with stable coenzymes
US9833183B2 (en) 2008-05-30 2017-12-05 Intuity Medical, Inc. Body fluid sampling device—sampling site interface
EP3639744B1 (en) 2008-06-06 2021-11-24 Intuity Medical, Inc. Blood glucose meter and method of operating
WO2009148624A1 (en) 2008-06-06 2009-12-10 Intuity Medical, Inc. Detection meter and mode of operation
AU2009269702B2 (en) 2008-07-11 2016-07-21 Universal Biosensors Pty Ltd Enhanced immunoassay sensor
EP2226007A1 (en) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Test element magazine with covered test fields
EP2226008A1 (en) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Method for producing an analytical magazine
EP2398909B1 (en) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Fast reaction kinetics of enzymes having low activity in dry chemistry layers
WO2010094427A2 (en) 2009-02-19 2010-08-26 Roche Diagnostics Gmbh Compact storage of auxiliary analytical devices in a cartridge
EP2292751A1 (en) 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisation of enzymes with stable coenzymes
WO2011065981A1 (en) 2009-11-30 2011-06-03 Intuity Medical, Inc. Calibration material delivery devices and methods
EP2362207A1 (en) * 2010-01-28 2011-08-31 F. Hoffmann-La Roche AG Measuring system and method, in particular for determining blood sugar
US9752990B2 (en) 2013-09-30 2017-09-05 Honeywell International Inc. Low-powered system for driving a fuel control mechanism
KR101530804B1 (en) 2011-04-12 2015-06-22 에프. 호프만-라 로슈 아게 Analytical aid
EP3750480B1 (en) 2011-08-03 2022-02-02 Intuity Medical, Inc. Body fluid sampling arrangement
EP2620507A1 (en) 2012-01-25 2013-07-31 Roche Diagnostics GmbH Method for the evaluation of the quality of a test element
KR101728597B1 (en) 2012-04-19 2017-04-19 에프. 호프만-라 로슈 아게 Method and device for determining an analyte concentration in blood
DE102013104906B4 (en) * 2013-05-13 2015-06-25 Brandenburgische Technische Universität Cottbus-Senftenberg Anhydrous immobilization of enzymes
JP2016528175A (en) 2013-06-04 2016-09-15 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Novel compounds useful for FRET and methods related thereto
US20170233787A1 (en) * 2014-08-05 2017-08-17 Becton, Dickinson And Company Methods and compositions for analyzing glucose-6-phosphate dehydrogenase activity in blood samples
KR102273051B1 (en) 2014-10-21 2021-07-06 삼성디스플레이 주식회사 Compound for hole transporting and organic light emitting device utilizing same
EP3339431A1 (en) 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
EP3856139A1 (en) * 2018-09-24 2021-08-04 Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V. Tailored layers of cellulose dispersions for detecting analytes
US11579093B2 (en) * 2020-04-22 2023-02-14 SciLogica Corp. Optical component

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3964974A (en) * 1972-09-28 1976-06-22 Merck Patent Gesellschaft Mit Beschrankter Haftung Enzymatic determination of glucose
US4451568A (en) * 1981-07-13 1984-05-29 Battelle Memorial Institute Composition for binding bioactive substances
US4820399A (en) * 1984-08-31 1989-04-11 Shimadzu Corporation Enzyme electrodes
US4919767A (en) * 1987-08-04 1990-04-24 Imperial Chemical Industries Plc Sensor and method for analyte determination
US5059526A (en) * 1982-09-06 1991-10-22 Konishiroku Photo Industry Co., Ltd. Dry multilayer analytical element for analysis of enzymes or triglycerides
US5340722A (en) * 1988-08-24 1994-08-23 Avl Medical Instruments Ag Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method
US5447847A (en) * 1993-09-02 1995-09-05 Nissui Pharmaceutical Co., Ltd. Quantitative determination of pyruvic acid and quantitative analysis for component of living body making use of such determination
US20080213808A1 (en) * 2007-02-27 2008-09-04 Wolfgang-Reinhold Knappe Mediators for photometric tests and means and methods relating to use thereof
US20080219809A1 (en) * 2003-11-10 2008-09-11 Van Der Meulen Peter Semiconductor manufacturing process modules
US7553615B2 (en) * 2005-07-28 2009-06-30 Roche Diagnostics Operations, Inc. Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
US20100227348A1 (en) * 2007-08-01 2010-09-09 Wolfgang Petrich Method and device for determining the concentration of an analyte using fluorescence measurement

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE638336A (en) * 1964-12-21 1964-02-03
US4238565A (en) 1978-06-22 1980-12-09 Miles Laboratories, Inc. Specific binding assay with a prosthetic group as a label component
DE2924249C2 (en) * 1978-06-22 1989-04-27 Miles, Inc., Elkhart, Ind., Us SPECIFIC BINDING INVESTIGATION METHOD FOR DETERMINING LIGAND IN A FLUID MEDIUM AND REAGENTS FOR CARRYING OUT THIS METHOD
AT367796B (en) * 1979-10-29 1982-07-26 List Hans METHOD FOR DETERMINING A SUBSTANCE CONCENTRATION IN A SOLUTION
DE3477849D1 (en) * 1983-12-06 1989-05-24 Ici Plc Dry film resists
GB8608435D0 (en) 1986-04-07 1986-05-14 Cranfield Inst Of Tech Specific binding assays
JPH0710896B2 (en) 1987-04-30 1995-02-08 第一工業製薬株式会社 Method for producing high molecular weight cationic acrylic polymer
CA1322324C (en) * 1988-02-10 1993-09-21 Joel M. Blatt Self-indicating and improved resolution analyses employing stoichiometric chemical subtraction
US5296305A (en) 1990-05-11 1994-03-22 Esslior International (Compagnie Generale D'optique) Method of fabricating a lens made of transparent polymer with modulated refracting index
GB9022304D0 (en) * 1990-10-15 1990-11-28 Ares Serono Res & Dev Ltd Assay technique
JP2977265B2 (en) * 1990-11-19 1999-11-15 旭化成工業株式会社 Photosensitive elastomer composition
DE4118880C2 (en) * 1991-06-08 1998-12-17 Fraunhofer Ges Forschung Method for the selective determination of the concentration of dissolved substances in enzymatic reactions
CA2100970A1 (en) * 1991-12-18 1993-06-19 Paul J. Buscemi Lubricous polyer network
DE59307720D1 (en) * 1992-03-23 1998-01-08 Siemens Ag Biosensor
JPH07115998A (en) 1993-08-31 1995-05-09 Iatron Lab Inc Reagent for got determination
JPH07115997A (en) 1993-08-31 1995-05-09 Iatron Lab Inc Reagent for gpt determination
GB9404805D0 (en) * 1994-03-11 1994-04-27 Minnesota Mining & Mfg Novel developing agents for (photo)thermographic systems
US5691205A (en) 1994-06-23 1997-11-25 Canon Kabushiki Kaisha Fluorometric analysis of chloride ion and chemical sensor therefor
JP2986712B2 (en) * 1995-05-01 1999-12-06 キヤノン株式会社 Chemical sensor, apparatus and method for measuring chloride ion concentration using the same
DE4424179C2 (en) * 1994-07-08 1998-09-10 Biotechnolog Forschung Gmbh UV-polymerizable enzyme paste for the production of thick-film biosensors and thick-film biosensors produced therewith
JPH09248200A (en) 1996-03-13 1997-09-22 Nissho Corp Reagent for assaying glucose
US5863650A (en) 1997-05-05 1999-01-26 Bioengineered Materials, Inc. Interfacial coatings
JP2000035413A (en) 1998-07-16 2000-02-02 Sapporo Imuno Diagnostic Laboratory:Kk Biosensor using dehydrogenase and coenzyme
AT409307B (en) 1999-01-12 2002-07-25 Hoffmann La Roche OPTICAL-CHEMICAL SENSOR
JP3869601B2 (en) 1999-11-26 2007-01-17 株式会社トクヤマ Method for measuring homocysteine
US6635412B2 (en) * 2000-07-11 2003-10-21 Martin A. Afromowitz Method for fabricating 3-D structures with smoothly-varying topographic features in photo-sensitized epoxy resists
WO2002052045A1 (en) 2000-12-26 2002-07-04 Aviva Biosciences Active and biocompatible platforms prepared by polymerization of surface coating films
US20030087178A1 (en) * 2001-04-20 2003-05-08 Adrian Lungu Photopolymerizable element for use as a flexographic printing plate and a process for preparing the plate from the element
US7341830B2 (en) * 2002-05-16 2008-03-11 Roche Diagnostics Operations, Inc. Method and reagent system having a non-regenerative enzyme-coenzyme complex

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3964974A (en) * 1972-09-28 1976-06-22 Merck Patent Gesellschaft Mit Beschrankter Haftung Enzymatic determination of glucose
US4451568A (en) * 1981-07-13 1984-05-29 Battelle Memorial Institute Composition for binding bioactive substances
US5059526A (en) * 1982-09-06 1991-10-22 Konishiroku Photo Industry Co., Ltd. Dry multilayer analytical element for analysis of enzymes or triglycerides
US4820399A (en) * 1984-08-31 1989-04-11 Shimadzu Corporation Enzyme electrodes
US4919767A (en) * 1987-08-04 1990-04-24 Imperial Chemical Industries Plc Sensor and method for analyte determination
US5340722A (en) * 1988-08-24 1994-08-23 Avl Medical Instruments Ag Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method
US5447847A (en) * 1993-09-02 1995-09-05 Nissui Pharmaceutical Co., Ltd. Quantitative determination of pyruvic acid and quantitative analysis for component of living body making use of such determination
US20080219809A1 (en) * 2003-11-10 2008-09-11 Van Der Meulen Peter Semiconductor manufacturing process modules
US7553615B2 (en) * 2005-07-28 2009-06-30 Roche Diagnostics Operations, Inc. Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
US20090246818A1 (en) * 2005-07-28 2009-10-01 Dieter Heindl Compounds, methods, complexes, apparatuses and uses relating to stabile forms of nad/nadh
US20080213808A1 (en) * 2007-02-27 2008-09-04 Wolfgang-Reinhold Knappe Mediators for photometric tests and means and methods relating to use thereof
US20100227348A1 (en) * 2007-08-01 2010-09-09 Wolfgang Petrich Method and device for determining the concentration of an analyte using fluorescence measurement

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