WO2024130577A1

WO2024130577A1 - Animal vital sign real-time monitoring system

Info

Publication number: WO2024130577A1
Application number: PCT/CN2022/140572
Authority: WO
Inventors: 王琳; 张一栏; 王烨
Original assignee: 中国科学院深圳先进技术研究院
Priority date: 2022-12-21
Filing date: 2022-12-21
Publication date: 2024-06-27

Abstract

An animal vital sign real-time monitoring system, comprising: a signal acquisition sensor (110), a wearable fabric (120), and a signal analysis platform (130). The signal acquisition sensor (110) is used for detecting physiological information parameters. The signal acquisition sensor (110) is fixed on the wearable fabric (120). The signal analysis platform (130) is used for acquiring the physiological information parameters and analyzing the physiological information parameters. The physiological information parameters comprise animal heart rate, respiratory rate, body temperature, blood oxygen saturation, and motion step number. The animal vital sign real-time monitoring system overcomes the defects of existing animal physiological information monitoring devices, such as system complexity, inability to be worn, single measurement parameter, and the need for in-vivo implantation, realizes the real-time monitoring of physiological information such as heart rate, respiratory rate, body temperature and blood oxygen of small animals during exercise without interfering with normal life activities of the small animals, and can monitor in real time and remotely record the changes in the vital sign data of mice.

Description

Real-time monitoring system for animal vital signs

Technical Field

The present application relates to the technical field of laboratory animal vital sign monitoring, and in particular to a real-time monitoring system for animal vital signs.

Background technique

The existing small animal wearable fabrics have a relatively simple function, usually used to fix small animals for acupuncture and other operations. Devices for monitoring the physiological information of small animals are generally complex, cannot be worn, and have few measurement parameters. There are implantable small animal physiological information monitoring technologies, but they will interfere with the normal physiological information of small animals.

At present, there are similar invention patents such as fixation clothes for mouse fixation and acupuncture. The patent application number 201420853966.5 "Fixation clothes and fixation device for experimental mice" proposes a mouse fixation clothes and fixation device used in medical animal experiments; the patent application number 201320727782.X "Mouse abdominal and dorsal acupuncture fixator" proposes a mouse abdominal and dorsal acupuncture fixator that can perform acupuncture on the back and abdomen of mice at the same time; the patent application number 200510019571.0 "Mouse asthma model vital signs monitoring device" realizes the accurate measurement and analysis of the vital signs of mouse asthma model, which is suitable for the basic research on the cause and pathogenesis of asthma. The measurement parameters of these devices are relatively single. Patent application number 201920519555.5, "A monitoring device for detecting the vital signs of mice", uses millimeter-wave radar and digital signal modules to analyze and process the heart rate and respiratory rate of mice, but the accuracy is not high; Patent application number 202111134089.7, "A laboratory anesthesia information processing system and method based on big data", collects corresponding data through electrocardiogram acquisition modules, blood pressure acquisition modules, and blood oxygen saturation acquisition modules, and processes anesthesia information by adjusting the concentration of anesthetic drugs. However, the system device and operation are relatively complicated. The above inventions for in vitro physiological information monitoring of mice have relatively simple measurement parameters and complex systems. At present, there are some small animal vital signs monitoring devices implanted in the body on the market, but they cause great damage to mice and affect normal physiological information parameters.

Summary of the invention

In view of this, it is necessary to provide a real-time animal vital signs monitoring system that can monitor the animal's physiological information in real time without interfering with the normal life activities of the animal in order to address the defects in the prior art.

To solve the above problems, this application adopts the following technical solutions:

One of the purposes of the present application is to provide a real-time monitoring system for animal vital signs, comprising: a signal acquisition sensor, a wearable fabric and a signal analysis platform, wherein the signal acquisition sensor is used to detect physiological information parameters, the signal acquisition sensor is fixed on the wearable fabric, and the signal analysis platform is used to obtain the physiological information parameters and analyze the physiological information parameters, wherein the physiological information parameters include animal heart rate, respiratory rate, body temperature, blood oxygen saturation and number of exercise steps.

In some of the embodiments, the information acquisition sensor is connected to the embedded main control STM32F103C8T6, and receives the physiological information parameters using a Bluetooth gateway through a broadcast protocol, and transmits the physiological information parameters to the signal analysis platform. The signal analysis platform obtains the animal's heart rate, respiratory rate, body temperature, blood oxygen saturation and exercise steps based on the physiological information parameters.

In some of the embodiments, the signal acquisition sensor includes a PPG green light module, and the PPG green light module obtains dynamic heart rate parameters according to photoplethysmography. The signal analysis platform performs time domain analysis on the dynamic heart rate parameters to calculate the heart rate value.

In some embodiments, the signal acquisition sensor includes a PPG red light and infrared light module. The PPG red light and infrared light module obtains the blood oxygen saturation parameter according to the different absorption characteristics of oxygenated hemoglobin HbO2 and hemoglobin Hb contained in the blood to light of different wavelengths, and the signal analysis platform calculates the corresponding ratio according to the blood oxygen saturation parameter to obtain the blood oxygen value.

In some of the embodiments, the signal acquisition sensor includes a high thermal conductivity temperature sensor, which can continuously measure the animal's body surface temperature, and the signal analysis platform calculates the body temperature through a built-in algorithm based on the body surface temperature.

In some embodiments, the signal acquisition sensor includes an accelerometer, using the a _x , a _y , and a _z parameters of the three-axis accelerometer, and the signal analysis platform obtains the signal through median filtering and peak detection.

Number of exercise steps.

In some of the embodiments, the signal analysis platform obtains the respiratory rate based on the heart rate value.

In some of the embodiments, the wearable fabric is made of elastic woven fabric, and a fixed pocket for fixing the signal acquisition sensor is provided at a position of the wearable fabric close to the heart of the animal.

This application adopts the above technical solution, and its beneficial effects are as follows:

The animal vital signs real-time monitoring system provided by the present application comprises: a signal acquisition sensor, a wearable fabric and a signal analysis platform, wherein the signal acquisition sensor is used to detect physiological information parameters, the signal acquisition sensor is fixed on the wearable fabric, and the signal analysis platform is used to obtain the physiological information parameters and analyze the physiological information parameters, wherein the physiological information parameters include the animal's heart rate, respiratory rate, body temperature, blood oxygen saturation and number of steps. The animal vital signs real-time monitoring system provided by the present application overcomes the defects of the existing animal physiological information monitoring devices, such as complex systems, inability to wear, single measurement parameters, and need for in vivo implantation, and realizes real-time monitoring of the heart rate, respiratory rate, body temperature, blood oxygen and other physiological information of the small animal during exercise without interfering with the normal life activities of the small animal. It can monitor and remotely record the changes in the vital signs data of mice in real time and obtain better data results.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

FIG1 is a schematic diagram of the structure of a real-time monitoring system for animal vital signs provided in an embodiment of the present application.

FIG2 is a schematic diagram of the structure of a wearable fabric provided in an embodiment of the present application.

FIG3 is a schematic diagram of a configuration page of the information analysis platform provided in Example 1 of the present application.

FIG4 is a schematic diagram of a system overview page of the information analysis platform provided in Example 1 of the present application.

FIG5 is a diagram showing the effect after the experiment provided in Example 1 of the present application.

Detailed ways

The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.

In the description of the present application, it should be understood that the terms "upper", "lower", "horizontal", "inside", "outside", etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

In order to make the objectives, technical solutions and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments.

Please refer to Fig. 1, which is a schematic diagram of the structure of a real-time monitoring system for animal vital signs provided by an embodiment of the present application, including: a signal acquisition sensor 110, a wearable fabric 120 and a signal analysis platform 130. The connection relationship between the various components and their implementation methods are described in detail below.

The signal acquisition sensor 110 is used to detect physiological information parameters, including animal heart rate, respiratory rate, body temperature, blood oxygen saturation and movement steps.

In this embodiment, the information acquisition sensor 110 is connected to the embedded main control STM32F103C8T6, and receives the physiological information parameters using a Bluetooth gateway through a broadcast protocol, and transmits the physiological information parameters to the signal analysis platform. The signal analysis platform obtains the animal's heart rate, respiratory rate, body temperature, blood oxygen saturation and exercise steps based on the physiological information parameters.

Please refer to FIG. 2 . The wearable fabric 120 is made of elastic woven fabric. A fixing pocket for fixing the signal acquisition sensor 110 is provided at a position of the wearable fabric 120 close to the animal's heart.

In some of the embodiments, the signal acquisition sensor 110 includes a PPG green light module, and the PPG green light module obtains dynamic heart rate parameters according to photoplethysmography. The signal analysis platform performs time domain analysis on the dynamic heart rate parameters to calculate the heart rate value.

In some embodiments, the signal acquisition sensor 110 includes a PPG red light and infrared light module. The PPG red light and infrared light module obtains the blood oxygen saturation parameter according to the different absorption characteristics of oxygenated hemoglobin HbO2 and hemoglobin Hb contained in the blood to light of different wavelengths, and the signal analysis platform calculates the corresponding ratio according to the blood oxygen saturation parameter to obtain the blood oxygen value.

In some embodiments, the signal acquisition sensor 110 includes a high thermal conductivity temperature sensor, which can continuously measure the animal's body surface temperature, and the signal analysis platform calculates the body temperature through a built-in algorithm based on the body surface temperature.

In some embodiments, the signal acquisition sensor 110 includes an accelerometer and a gyroscope, using _ax , _ay , _az of the accelerometer and _mx , _my , _mz of the gyroscope, and the signal analysis platform 130 obtains the number of motion steps by using median filtering and peak detection.

In some embodiments, the signal analysis platform 130 obtains the respiratory rate based on the heart rate value.

The real-time monitoring system for animal vital signs provided by the above-mentioned embodiment of the present application, the signal acquisition sensor 110 is used to detect physiological information parameters, the signal acquisition sensor 110 is fixed on the wearable fabric 120, and the signal analysis platform 130 is used to obtain the physiological information parameters and analyze the physiological information parameters, and the physiological information parameters include animal heart rate, respiratory rate, body temperature, blood oxygen saturation and movement steps. The real-time monitoring system for animal vital signs provided by the present application overcomes the defects of the existing animal physiological information monitoring device, such as complex system, inability to wear, single measurement parameters, and need for in vivo implantation, and realizes real-time monitoring of the heart rate, respiratory rate, body temperature, blood oxygen and other physiological information of small animals during exercise without interfering with the normal life activities of small animals. It can monitor and remotely record the changes in the vital signs data of mice in real time, and obtain better data results.

The technical solution provided in the above embodiment of the present application is described in detail below in conjunction with specific embodiment 1.

This example uses mice as an example. 6-8 week old healthy white mice are suspended at 5° using the tail suspension method (with the hind limbs unloaded). The mice can grow and move normally.

In this embodiment, the device consists of three parts: wearable fabric, signal acquisition sensor (mainly optical sensor) and physiological information acquisition and analysis system. (See Figure 1 and Figure 2)

The purpose of the wearable clothing is to fix the signal acquisition sensor, read the dynamic heart rate, respiratory rate, body temperature, blood oxygen saturation SPO2 and other data; and at the same time, the mouse can move freely. Therefore, the wearable clothing uses elastic fabric and the size is made according to the body size of the experimental mouse. Leave a hole for the mouse's forelimbs so that the mouse can move freely. In the position of the wearable clothing close to the mouse's heart, leave a fixed pocket for the sensor and a hole for detecting the signal so that the signal can be collected normally. The information acquisition sensor is connected to the embedded main control. STM32F103C8T6, and uses the Bluetooth gateway to receive data through the broadcast protocol. The data is developed into our physiological information acquisition system platform, and the heart rate, respiratory rate, body temperature, blood oxygen saturation SPO2, etc. of the mouse are calculated through relevant algorithms.

The specific implementation method is as follows: the dynamic heart rate is measured by the PPG green light module with an accuracy of 5bpm, 10bpm and 15bpm, and the data is broadcast once every 250ms. The power consumption is ultra-low and accurate, supporting broadcast protocol reading and SDK/API APP reading. According to the photoplethysmography method, due to the blood flow in the artery, the absorption of light changes, and the obtained signal is divided into a DC signal and an AC DC signal. The heart rate value is calculated through time domain analysis. According to the distribution of the left coronary artery on the ventral surface of the mouse heart, a flexible signal acquisition device is designed with a size of 24mm long and 10mm wide.

Blood oxygen SPO2 is measured by PPG red light and infrared light modules, with high power consumption of about 800uA. Since oxygenated hemoglobin HbO2 and hemoglobin Hb contained in the blood have different absorption characteristics for light of different wavelengths, the signal analysis platform calculates the corresponding ratio through relevant algorithms to obtain the blood oxygen value.

The surface temperature is measured by a high thermal conductivity temperature sensor, which continuously measures the surface temperature of the mouse and broadcasts it continuously. The accuracy is ±0.1 degrees Celsius. The signal analysis platform calculates the body temperature by combining the surface temperature with the built-in algorithm. The signal analysis platform calculates the respiratory rate in combination with the measured heart rate.

The number of steps is mainly obtained by using a _x , a _y , a _z of the accelerometer and m _x , my _y , m _z of the gyroscope, and by using median filtering and peak detection. The sampling rate is 25 Hz.

The measured data is uploaded to the physiological information monitoring system via the Bluetooth connection protocol. When using the system, the first step is to configure the age and weight of the user (i.e., the mouse) and connect the sensor device, as shown in Figure 3, which is a schematic diagram of the configuration page of the information analysis platform provided in this embodiment.

The second step is to enter the system overview page and start collecting mouse physiological information, as shown in FIG4 , which is a schematic diagram of the system overview page provided in this embodiment.

The third step is to enter the detailed parameter page and observe the real-time data. The heart rate, respiratory rate, and body surface temperature output results every 30 seconds to form a line graph; the blood oxygen output measurement results every 2 minutes and then draw a bar graph.

Specific implementation method: The experimental mouse vital signs monitoring is composed of a non-restrained wearable clothing, a sensor, an experimental mouse, a vital signs observation cabin, and an electronic computer. The experimental mouse is suspended.

Please refer to FIG5 , which is a diagram showing the effect of the experiment in the embodiment of the present application. The heart rate of the experimental mice can be accurately measured and recorded, and it can be seen that the result meets the expectations and requirements.

The results show that the present invention has designed a real-time monitoring system for animal vital signs, which can record parameters such as heart rate, respiratory rate, body temperature, blood oxygen saturation SPO2, etc. during the unrestrained movement of small animals, thereby facilitating the exploration of changes in relevant physiological information of small animals during normal life activities.

It can be understood that the technical features of the above-described embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

The above are only preferred embodiments of the present application, and only specifically describe the technical principles of the present application. These descriptions are only for explaining the principles of the present application and cannot be interpreted as limiting the scope of protection of the present application in any way. Based on the explanation here, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application, and other specific implementation methods of the present application that can be associated with the technicians in this field without creative work, should be included in the scope of protection of the present application.

Claims

A real-time monitoring system for animal vital signs, characterized in that it includes: a signal acquisition sensor, a wearable fabric and a signal analysis platform, wherein the signal acquisition sensor is used to detect physiological information parameters, the signal acquisition sensor is fixed on the wearable fabric, and the signal analysis platform is used to obtain the physiological information parameters and analyze the physiological information parameters, wherein the physiological information parameters include animal heart rate, respiratory rate, body temperature, blood oxygen saturation and exercise steps.
The real-time monitoring system for animal vital signs as described in claim 1 is characterized in that the information acquisition sensor is connected to the embedded main control STM32F103C8T6, and receives the physiological information parameters using a Bluetooth gateway through a broadcast protocol, and transmits the physiological information parameters to the signal analysis platform, and the signal analysis platform obtains the animal's heart rate, respiratory rate, body temperature, blood oxygen saturation and number of exercise steps according to the physiological information parameters.
The real-time monitoring system for animal vital signs as described in claim 1 or 2 is characterized in that the signal acquisition sensor includes a PPG green light module, the PPG green light module obtains dynamic heart rate parameters according to photoplethysmography, and the signal analysis platform performs time domain analysis on the dynamic heart rate parameters to calculate the heart rate value.
The real-time monitoring system for animal vital signs according to claim 1 or 2 is characterized in that the signal acquisition sensor includes a PPG red light and infrared light module. The PPG red light and infrared light module obtains the blood oxygen saturation parameter according to the different absorption characteristics of oxygenated hemoglobin HbO2 and hemoglobin Hb contained in the blood to light of different wavelengths, and the signal analysis platform calculates the corresponding ratio according to the blood oxygen saturation parameter to obtain the blood oxygen value.
The real-time monitoring system for animal vital signs as described in claim 1 or 2 is characterized in that the signal acquisition sensor includes a high thermal conductivity temperature sensor, the high thermal conductivity temperature sensor can continuously measure the animal's body surface temperature, and the signal analysis platform calculates the body temperature through a built-in algorithm based on the body surface temperature.
The real-time monitoring system for animal vital signs according to claim 1 or 2, characterized in that the signal acquisition sensor includes an accelerometer and a gyroscope, using a x , a y , a z of the accelerometer and m x , my , m z of the gyroscope, and the signal analysis platform uses median filtering and peak detection to obtain the number of movement steps.
The real-time monitoring system for animal vital signs as described in claim 3 is characterized in that the signal analysis platform obtains the respiratory rate based on the heart rate value.
The real-time monitoring system for animal vital signs as described in claim 1 is characterized in that the wearable fabric is made of elastic woven fabric, and a fixed pocket for fixing the signal acquisition sensor is provided at a position of the wearable fabric close to the animal's heart.