CN110244035A - The readable detection device of immunochromatographic method continuous and quantitative - Google Patents
The readable detection device of immunochromatographic method continuous and quantitative Download PDFInfo
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- CN110244035A CN110244035A CN201910385920.2A CN201910385920A CN110244035A CN 110244035 A CN110244035 A CN 110244035A CN 201910385920 A CN201910385920 A CN 201910385920A CN 110244035 A CN110244035 A CN 110244035A
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- detection device
- opening
- immunochromatography
- readable
- cavity structure
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- 238000001514 detection method Methods 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title abstract description 4
- 238000012360 testing method Methods 0.000 claims abstract description 19
- 239000000126 substance Substances 0.000 claims abstract description 9
- 238000003317 immunochromatography Methods 0.000 claims description 21
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- -1 cyclic ester Chemical class 0.000 claims description 12
- 150000002391 heterocyclic compounds Chemical class 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 10
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 150000003950 cyclic amides Chemical class 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical group ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 claims description 3
- 125000004429 atom Chemical group 0.000 claims description 3
- 229960002685 biotin Drugs 0.000 claims description 3
- 235000020958 biotin Nutrition 0.000 claims description 3
- 239000011616 biotin Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 125000004430 oxygen atom Chemical group O* 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000012472 biological sample Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The embodiment of the invention provides a kind of readable detection devices of immunochromatographic method continuous and quantitative, comprising: encapsulating structure and detection module；Wherein, the encapsulating structure is integral type cavity structure, is provided with opening at the top of the cavity structure；The detection module is arranged inside the cavity structure by the opening, and the detection module is immunochromatographytest test piece, is provided with a plurality of default lines on the immunochromatographytest test piece, and a plurality of default lines respectively correspond different numerical value.It will test module to be encapsulated in the cavity structure of opening, determinand can be detected by detection module by opening opening when detecting, the apparatus structure is simple, detection method step is easy, it is easily operated, and can the concentration for detecting test substance quick and precisely, quantitative, can quickly measure the content of substance to be checked in sample.
Description
Technical Field
The embodiment of the invention relates to the technical field of biological detection, and particularly relates to an immunochromatography continuous quantitative readable detection device.
Background
In the immunochromatography, a biological sample is developed while moving within a porous support body by capillary action from one end of the porous support body on which an antibody specifically binding to a substance to be detected is immobilized, and in the process, the labeled substance to be detected and the antibody are bound by immunoreaction, and then aggregate over time, and develop color locally, thereby determining the presence or absence of the substance to be detected in the biological sample.
Since the technology appears, the development is very rapid, the application is more and more extensive, and the technology can be applied to various research fields of biomedicine, and is particularly more commonly used in medical inspection. While conventional immunochromatography is generally performed by visual determination (qualitative evaluation), in recent years, a technique has been developed in which the concentration of a substance to be detected contained in a biological sample is quantified by using a device such as a chromatographic reader for measuring the intensity of color development.
Many quantitative detection technologies also appear at present, but the corresponding detection device has a complex structure and complex detection steps, and cannot quickly and accurately detect the concentration of an object to be detected.
Disclosure of Invention
Embodiments of the present invention provide an immunochromatographic continuously quantitatively readable detection device that overcomes or at least partially solves the above-mentioned problems.
The embodiment of the invention provides an immunochromatography continuous quantitative readable detection device, which comprises: the device comprises a packaging structure and a detection module; wherein,
the packaging structure is an integrated cavity structure, and an opening is formed in the top of the cavity structure;
the detection module passes through the opening sets up inside the cavity structure, the detection module is the immunochromatography test piece, be provided with many on the immunochromatography test piece and predetermine the lines, just many are predetermine the lines and are corresponded different numerical values respectively.
Furthermore, the shape bit of the cavity structure is longer than the cuboid, and the opening is arranged on one side surface of the cuboid.
Furthermore, one surface of the cuboid, which is opposite to the immunochromatography test strip, is made of a transparent material and is used for reading the immunochromatography test strip in real time.
Further, the immunochromatographic test strip comprises a conjugate pad and a pad membrane; wherein
The pad membrane is impregnated with a first reagent combined with an object to be detected and used for combining with a specific substance in the object to be detected to obtain a combined product;
the pad membrane is impregnated with a second reagent for binding to the binding product and displaying a different color to indicate a reading.
Further, the first reagent is nitrous acid generated on the pad film by a contact reaction of a nitrite and a heterocyclic compound having at least one skeleton selected from the group consisting of a cyclic ester, a cyclic amide, and a cyclic imide.
Further, the heterocyclic compound having a skeleton of a cyclic ester is a 5-membered ring compound having 1 to 2 oxygen atoms.
Further, the heterocyclic compound having a skeleton of a cyclic amide and the heterocyclic compound having a skeleton of a cyclic imide are 5-or 6-membered ring compounds having 1 to 3N atoms.
Further, the second reagent comprises detection particles to which a biotin-labeled protein is bound.
According to the continuous quantitative readable detection device for immunochromatography provided by the embodiment of the invention, the detection module is packaged in the cavity structure with the opening, and the object to be detected can be detected through the detection module by opening the opening during detection.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly described below, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The continuous quantitative readable detection device of the immunochromatography provided by the embodiment of the invention comprises: the device comprises a packaging structure and a detection module; wherein,
the packaging structure is an integrated cavity structure, and an opening is formed in the top of the cavity structure;
the detection module passes through the opening sets up inside the cavity structure, the detection module is the immunochromatography test piece, be provided with many on the immunochromatography test piece and predetermine the lines, just many are predetermine the lines and are corresponded different numerical values respectively.
According to the continuous quantitative readable detection device for immunochromatography provided by the embodiment of the invention, the detection module is packaged in the cavity structure with the opening, and the object to be detected can be detected through the detection module by opening the opening during detection.
In the above embodiment, the outer shape of the cavity structure is longer than the cuboid, and the opening is disposed on one side surface of the cuboid.
In the above embodiment, one surface of the rectangular parallelepiped facing the immunochromatographic test strip is made of a transparent material, and is used for reading the immunochromatographic test strip in real time.
In the above embodiment, the immunochromatographic test strip comprises a conjugate pad and a pad membrane; wherein
The pad membrane is impregnated with a first reagent combined with an object to be detected and used for combining with a specific substance in the object to be detected to obtain a combined product;
the pad membrane is impregnated with a second reagent for binding to the binding product and displaying a different color to indicate a reading.
In the above embodiment, the first reagent is nitrous acid generated on the pad film by a contact reaction of a nitrite and a heterocyclic compound having at least one skeleton selected from the group consisting of a cyclic ester, a cyclic amide, and a cyclic imide.
In the above embodiments, the heterocyclic compound having a skeleton of a cyclic ester is a 5-membered ring compound having 1 to 2 oxygen atoms.
In the above-described embodiments, the heterocyclic compound having a skeleton of a cyclic amide and the heterocyclic compound having a skeleton of a cyclic imide are 5-or 6-membered ring compounds having 1 to 3N atoms.
In the above embodiment, the second reagent comprises a detection particle to which a biotin-labeled protein is bound.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (8)
1. An immunochromatography continuous quantitative readable detection device, comprising: the device comprises a packaging structure and a detection module; wherein,
the packaging structure is an integrated cavity structure, and an opening is formed in the top of the cavity structure;
the detection module passes through the opening sets up inside the cavity structure, the detection module is the immunochromatography test piece, be provided with many on the immunochromatography test piece and predetermine the lines, just many are predetermine the lines and are corresponded different numerical values respectively.
2. The continuous quantitative readable detection device for immunochromatography according to claim 1, wherein the outer shape of the cavity structure is longer than a rectangular body, and the opening is provided on one side of the rectangular body.
3. The continuous quantitative readable detection device for immunochromatography according to claim 2, wherein one surface of the rectangular parallelepiped facing the immunochromatography test strip is made of a transparent material and is used for reading the immunochromatography test strip in real time.
4. The immunochromatographic continuous quantitative readable detection device according to claim 1, wherein the immunochromatographic test strip comprises a conjugate pad and a pad membrane; wherein
The pad membrane is impregnated with a first reagent combined with an object to be detected and used for combining with a specific substance in the object to be detected to obtain a combined product;
the pad membrane is impregnated with a second reagent for binding to the binding product and displaying a different color to indicate a reading.
5. The immunochromatographic continuous quantitative readable detection device according to claim 4, wherein the first reagent is nitrous acid generated on the pad membrane by a contact reaction of a heterocyclic compound having at least one skeleton selected from the group consisting of a cyclic ester, a cyclic amide and a cyclic imide with nitrite.
6. The immunochromatographic continuous quantitative readable detection device according to claim 5, wherein the heterocyclic compound having a skeleton of a cyclic ester is a 5-membered ring compound having 1 to 2 oxygen atoms.
7. The immunochromatographic continuous quantitative readable detection device according to claim 5, wherein the heterocyclic compound having a skeleton of a cyclic amide and the heterocyclic compound having a skeleton of a cyclic imide are 5-or 6-membered ring compounds having 1 to 3N atoms.
8. The continuous quantitative readable detection device of claim 4, wherein the second reagent comprises detection particles to which biotin-labeled protein is bound.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910385920.2A CN110244035A (en) | 2019-05-09 | 2019-05-09 | The readable detection device of immunochromatographic method continuous and quantitative |
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| CN201910385920.2A CN110244035A (en) | 2019-05-09 | 2019-05-09 | The readable detection device of immunochromatographic method continuous and quantitative |
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Citations (7)
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| US20050181517A1 (en) * | 1999-03-11 | 2005-08-18 | Enterix Pty. Limited, A Sydney, Australia Corporation | Sample collection and testing system |
| US20060160170A1 (en) * | 2004-12-21 | 2006-07-20 | Paolo Giordano | Method and device of rapid antigen extraction |
| CN1879017A (en) * | 2002-12-26 | 2006-12-13 | 梅索斯卡莱科技公司 | Methods, compositions and kits for biomarker extraction |
| CN101915833A (en) * | 2010-09-10 | 2010-12-15 | 中国科学院东北地理与农业生态研究所 | Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof |
| CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
| CN107850593A (en) * | 2015-07-16 | 2018-03-27 | 田中贵金属工业株式会社 | Method of immunity, immune chromatography reagent kit |
| CN108139395A (en) * | 2015-10-16 | 2018-06-08 | 东洋纺株式会社 | Immunochromatographytest test piece |
-
2019
- 2019-05-09 CN CN201910385920.2A patent/CN110244035A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050181517A1 (en) * | 1999-03-11 | 2005-08-18 | Enterix Pty. Limited, A Sydney, Australia Corporation | Sample collection and testing system |
| CN1879017A (en) * | 2002-12-26 | 2006-12-13 | 梅索斯卡莱科技公司 | Methods, compositions and kits for biomarker extraction |
| US20060160170A1 (en) * | 2004-12-21 | 2006-07-20 | Paolo Giordano | Method and device of rapid antigen extraction |
| CN101915833A (en) * | 2010-09-10 | 2010-12-15 | 中国科学院东北地理与农业生态研究所 | Semi-quantitative rapid detection test paper for immunoglobulin G in bovine colostrum and products thereof and preparation method thereof |
| CN107850593A (en) * | 2015-07-16 | 2018-03-27 | 田中贵金属工业株式会社 | Method of immunity, immune chromatography reagent kit |
| CN108139395A (en) * | 2015-10-16 | 2018-06-08 | 东洋纺株式会社 | Immunochromatographytest test piece |
| CN106526178A (en) * | 2016-12-08 | 2017-03-22 | 刘宏川 | Continuous quantitative readable detection device adopting immunochromatography |
Non-Patent Citations (2)
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Application publication date: 20190917 |