CN106526178A - Continuous quantitative readable detection device adopting immunochromatography - Google Patents
Continuous quantitative readable detection device adopting immunochromatography Download PDFInfo
- Publication number
- CN106526178A CN106526178A CN201611123646.4A CN201611123646A CN106526178A CN 106526178 A CN106526178 A CN 106526178A CN 201611123646 A CN201611123646 A CN 201611123646A CN 106526178 A CN106526178 A CN 106526178A
- Authority
- CN
- China
- Prior art keywords
- pad
- sample
- detection means
- readable
- quantitative
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Dispersion Chemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a continuous quantitative readable detection device adopting immunochromatography. The continuous quantitative readable detection device comprises an upper casing, a lower casing cooperating with the upper casing to form an accommodating chamber as well as a test strip arranged in the accommodating chamber, wherein the test strip comprises a liner board as well as a conjugate pad, a reaction pad and an absorption pad which are in sequential lap joint on the liner board, a quality control line and a detection region belt are arranged sequentially on the reaction pad in the direction of the conjugate pad pointing to the absorption pad, and the detection region belt is coated with an antigen or an antibody corresponding to a detected substance. The continuous quantitative readable detection device is simple in structure, a detection method comprises simple steps and is easy to operate, the concentration of the to-be-detected substance can be detected rapidly, accurately and quantitatively, and the content of the to-be-detected substance in a sample can be determined rapidly.
Description
Technical field
The present invention relates to technical field of biological, more particularly to a kind of immunochromatographic method continuous and quantitative is readable to detect dress
Put.
Background technology
Immunochromatographic method (immunochromatography) is a kind of quick diagnosis technology of external rise in recent years,
Its principle is a certain zone that special antibody is first fixed on nitrocellulose filter, when celluloid one end of the drying is soaked
After entering sample (various Fluids and secretions), due to capillarity, sample will be moved forward along the film, when being moved to fixation
When having the region of antibody, in sample there is specific binding with the antibody in corresponding antigen, if with immune colloid gold, immunoenzyme
Dyeing or immune colored latex particles can make the region show certain color, so as to realize the immunologic diagnosises of specificity.
Develop since occurring from the technology very rapidly, application also more and more extensively, almost can be applicable to biological doctor at present
Each research field is learned, especially obtains more universal used in medical test.The technology is through use for many years, it is shown that numerous
Advantage：Operation is simple, without the need for special installation, nontoxic pollution-free etc.；There is also certain inferior position：It is difficult quantitative.
Conventional immune chromatography method is qualitative or sxemiquantitative in the market so that result is the judgement or quantitative whetheing there is
As a result it is a scope, so that not accurate enough rigorous；And the method for accurate detection by quantitative includes chemoluminescence method, time
Resolved fluorometric method etc., these procedures are complicated, cumbersome, need to be by corresponding analysis and detecting instrument, and time-consuming；Automatically
Though the precision detecting instrument quick detection that can realize to large sample amount, the Expenses Cost of instrument and reagent is at a relatively high, nothing
Method makes the method for Quantitative detection be conveniently applied to conventional sense, and penetration and promotion is more unable to reach the independent detection of individual
With the purpose of real-time easy detection disease.For numerous detectable, (people promotees for such as HCG (human chorionic gonadotropin), LH
Luteotropic hormone) etc. hormone series；Tumor series such as AFP (alpha-fetoprotein), PSA (prostate specific antigen) etc., its inspection
Survey the positive height meaning of result to differ greatly, represent the different phase of different diseases or disease.Therefore, realize quantitative
That what is detected is significant.
Therefore, in order to make up prior art presence drawbacks described above, it is necessary to a kind of immunochromatographic method continuous and quantitative is readable
Detection means, can quickly determine the content of material to be checked in sample.
The content of the invention
In view of this, it is an object of the invention to provide a kind of readable detection means of immunochromatographic method continuous and quantitative, can
The quick content for determining material to be checked in sample.
The readable detection means of immunochromatographic method continuous and quantitative of the present invention, including upper shell and upper shell cooperatively form appearance
The lower house and the test strips located at accommodation chamber of room are put, the test strips include liner plate and are located at the combination for being overlapped on liner plate successively
Pad, reacting pad and absorption pad, the direction for pointing to absorption pad along pad on the reacting pad are sequentially provided with nature controlling line and detection zone
Domain band, the detection zone band are coated with antigen corresponding with thing to be checked or antibody.
Further, the upper shell is provided with the observation for observing detection zone band colour developing situation corresponding with reacting pad
Hole, the edge of the observation port are provided with the concentration scale line for showing measured object content corresponding with detection zone band.
Further, the accommodation chamber is additionally provided with sample pad, and the rear end of the sample pad is contacted with pad, the upper casing
Body is provided with the well for adding sample corresponding with sample pad.
Further, the upper shell is provided with the chromatography developing solvent hole for adding developing solvent corresponding with sample pad front end.
Further, the bottom of the well is provided with the lower surface of sample seperation film, the sample pad and sample seperation film
Contact.
Further, this device is provided with least two test strips；The pad of each test strips with sample pad after
End contacts.
Further, pad is set above described liner plate one end, absorption pad, sample pad rear end is set above the liner plate other end
Lower section is overlapped with the blank end of pad, is reacting pad between pad and absorption pad.
Further, the upper shell is hinged with one end of lower house, the other end is connected by buckle.
Further, the reacting pad is cellulose acetate film pad or nitrocellulose membrane pad.
Further, the pad is latex mattress or nanometer gold pad.
Beneficial effects of the present invention：The readable detection means of immunochromatographic method continuous and quantitative of the present invention, due to test strips
Detection zone band is provided with reacting pad, detection zone band contains even concentration distribution and sets up the detection of certain range of linearity
Thing, according to the saturability principle of the immunoreation of antigen-antibody, in tandem reaction sequence, gradually colour developing decrease from depth to shallow is
To not developing the color for reaction end, the continuous readable laboratory detection by quantitative effect of immunoreation has been reached；The structure of the present invention
Simply, detection method step is easy, it is easy to operate, and can quick and precisely, quantitative the concentration for detecting test substance, energy
Enough quick contents for determining material to be checked in sample.
Description of the drawings
The invention will be further described with reference to the accompanying drawings and examples：
Fig. 1 is the structural representation of the first structure of the invention；
Fig. 2 is the structural representation of second structure of the invention；
Fig. 3 is A-A sectional views in Fig. 1；
Fig. 4 is the structural representation of the test strips of the present invention.
Specific embodiment
Embodiment one
As shown in Figures 1 to 4：The readable detection means of immunochromatographic method continuous and quantitative of the present embodiment, including upper shell 1,
The lower house 2 and the test strips located at accommodation chamber of accommodation chamber are cooperatively formed with upper shell 1, the test strips include liner plate 31 and set
Pad 32, reacting pad 33 and the absorption pad 34 for being overlapped on liner plate 31 successively, points to along pad 32 on the reacting pad 33
The direction of absorption pad 34 is sequentially provided with a nature controlling line 33a and detection zone band 33b, and the detection zone band 33b is coated with one
Determine concentration and equally distributed antigen corresponding with thing to be checked or antibody；Detection zone band 33b is coated with corresponding with thing to be checked
Antigen or antibody, this is the basis for realizing immunoreation saturability principle；The concentration of antigen or antibody determines the detection means
Linear and sensitivity；Upper shell 1 is used to cover lower house 2, forms certain protective effect；Upper shell 1, lower house 2
Made using medical plastic, upper shell 1 is preferably transparent configuration；Upper shell 1 can be connected in several ways with lower house 2, example
It is hinged with one end of lower house 2 such as the upper shell 1, the other end is connected by buckle, is easy to control upper shell 1 be opened and closed；Examination
Paper slip is used for the detection by quantitative of various humoral samples and secretions, and in sample, all reactions that can be adapted to antigen-antibody is all to be checked
The detection of thing, including the antagonism of the antibody of virus antigen or generation, the metabolite of antibacterial and its antagonist, hormone and its generation
Antagonist of thing, the content of tumor marker, the use of medicine and its generation etc.；Pad 32 is set above 31 one end of liner plate,
Absorption pad 34 is set above 31 other end of liner plate, with the blank end of pad 32 (i.e. without relevant particles below sample pad rear end
Part) overlap, between pad 32 and absorption pad 34 be reacting pad 33；Liner plate 31 can be polystyrene component or polyethylene
Component, plays support effect；It (can for example be card that the upper surface of lower house 2 is provided with 31 fixed part of liner plate for fixing liner plate 31
Slot structure)；Pad 32 can be latex mattress or nanometer gold pad (including colloidal gold pad), contain on colloidal gold pad or latex particle pad
There are the antigen or antibody of quantitative colloid gold particle or latex particle combination, this quantitative setting is to realize that immunoreation saturability is former
The basis of reason；Absorption pad 34 can be made up of absorbent filter, provide the power of sample flow by capillarity；Nature controlling line 33a is used
In the effectiveness of test strip, determine whether reaction system is set up；Reacting pad 33 can be that cellulose acetate film pad or nitric acid are fine
Dimension film pad；As detection zone band 33b is provided with the reacting pad 33 of test strips, detection zone band 33b contains even concentration point
Cloth and the detectable substance of certain range of linearity is set up, according to the saturability principle of the immunoreation of antigen-antibody, in successive reaction
During gradually colour developing weaken from depth to shallow or even do not develop the color for reaction end, reached the continuous readable experiment of immunoreation
Room detection by quantitative effect；The simple structure of the present invention, detection method step are easy, it is easy to operate, and can quick and precisely, calmly
The concentration for detecting test substance of amount, can quickly determine the content of material to be checked in sample.
In the present embodiment, the upper shell 1 is provided with corresponding with reacting pad 33 for observing detection zone band 33b colour developings
The observation port 11 of situation, the edge of the observation port 11 are provided with corresponding for showing measured object content with detection zone band 33b
Concentration scale line 11a；When there is material to be checked in sample, color change should be it is from depth to shallow or even colourless, it is this moment right
According to graduation mark, the indicating graduation of the junction as ultimate density of thing to be checked with colour developing not developed the color with 33b in detection zone
And it is readable；The accommodation chamber is additionally provided with sample pad 4, and the rear end of the sample pad 4 is contacted with pad 32, the upper shell 1
It is provided with the well 12 for adding sample corresponding with sample pad 4；Well 12 can be engaged with existing sample loading gun, added
The testing sample of determined volume can be added in sample hole 12, this setting is also the basis for realizing immunoreation saturability principle；It is described
Upper shell 1 is provided with the chromatography developing solvent hole 13 for adding developing solvent corresponding with 4 front end of sample pad；The bottom of the well 12
Portion is provided with sample seperation film 5, and the sample pad 4 is contacted with the lower surface of sample seperation film 5；Sample seperation film 5 is conducive to sample
The filtration of product, especially for the filtration of the interference components such as cell；As described in Figure 1, a test strips can be set in accommodation chamber；
Can be as shown in Fig. 2 three (can actually arrange at least two) test strips, the knot of now each test strips be provided with accommodation chamber
Close pad 32 to contact with the rear end of sample pad 4；In order to the inspection of multiple projects of correlation can be carried out in same device
Survey.
The device make use of the saturability principle of the immunoreation immobilized antigen in a fixed reaction system to resist
The linear content of body, the scale indicated by developed width reacted in detection zone band according to specimen is completing detection by quantitative；It is real
The conjugate of the antigen or antibody of the gold colloidal or latex particle of border setting fixed amount is continuous with setting conversion zone detection band
Property be the basis for realizing continuous and quantitative detection, arrange quantitative sample-adding detecting in linear scope to set；Realize continuous
Detection is quantitative, and this 3 points is necessary.
Embodiment two
Using hepatitis B surface antibody (HBs-Ag) content in the device detection blood of human body in embodiment one：
In blood of human body hepatitis B surface antibody (HBs-Ag) normal condition be can not be detected or negative findings or fixed
Amount method detects its value<1U/ml, so range of linearity 50U/ml of initial setting detection zone, according to the needs of experimental design, inspection
The range of linearity for surveying area (region that i.e. detection line is located) can also set different numerical value.
Antigen antibody reaction principle and latex mark immunity-chromatography technology double antibody folder of this detection using high special
Heart method.
During detection, a kind of quantitative whole blood (proportioning device) is added in well 12, in chromatography developing solvent hole after the 5-10 seconds
13 add quantitative developing solvent, and under the capillary tension effect of developing solvent, the blood plasma being diluted is moved to detection zone direction, if
It is positive sample, then the antibody response of the Anti-HBsAg antibody-Ag first combined with pre-coated latex particle by HBs-Ag generates conjugate, should
Conjugate or unconjugated latex particle conjugate are travelled forward therewith, nature controlling line 33a colour developings, represent that detection is set up, detection zone
Nitrocellulose membrane on coating and reacted by the antibody of fixed Anti-HBsAg antibody-Ag and latex particle conjugate, form HBs-Ag and resist
Body -- the complex of HBs-Ag--HBs-Ag antibody, and start aobvious red color area band occur, color is gradually become shallower as until not showing forward
Color, now colour developing and the terminal that the cross point do not developed the color is exactly reaction, corresponding concentration scale is exactly the concrete content of HBs-Ag.
If HBs-Ag is not contained in specimen, detection zone would not develop the color, and be indicated as feminine gender.But nature controlling line 33aC is regardless of any
Situation will develop the color, and otherwise be treated as reagent failure.
For example：Quantitative whole blood is added toward well 12, after the 5-10 seconds, quantitative developing solvent is added, is then observed, 10
After many minutes, it was observed that, detection zone colour developing stops, and this corresponding concentration scale of point is 23U/ml, and front has not been developed the color, then may be used
To draw, the HBs-Ag contents in blood are 23U/ml.
Embodiment three
Using trachoma chlamydia antigen (CT-Ag) in the device detection secretions in embodiment one.
Chlamydia trachomatiss parasitize intracellular, are divided three classes by its characteristic, have 15 serotypes, wherein having 12 and trachoma
Relevant with the infection of reproductive tract, 3 relevant with the generation of lymphogranuloma venereum.This law adopts colloidal gold method, is to use anti-clothing
Substance lipopolysaccharide monoclonal antibody and sheep anti-mouse igg polyclonal antibody are individually fixed on solid phase nitrocellulose membrane, using dual anti-
The form of body sandwich assay sets up the detection by quantitative mode of Chlamydia antigen.For chlamydia trachomatiss in the various secretions of human body
Detection.
The secretions of acquirement are put in ready sample tube, Deca dissolving fixative A, B liquid, stirring and evenly mixing 10 seconds,
Upper shell 1 is covered, this process identifier specimen is ready.
Ready specimen is dripped in 5 drops and well 12, after the exhibition that 13 Deca of chromatography developing solvent hole are quantitative in 10 seconds
Agent is opened, starts test, the Chlamydia antigen in sample is first combined with the anti-chlamydial LPS monoclonal antibody of colloid gold label,
This conjugate launches with capillarity forward, colour developing is first combined with the sheep anti-mouse igg antibody on nature controlling line 33a and (represents detection
Set up), expansion is continued thereafter with, with anti-- II shape of chlamydial LPS monoclonal antibody fixed on the nitrocellulose membrane of detection zone
Develop the color into compound compound.
Finally illustrate, above example is only unrestricted to illustrate technical scheme, although with reference to compared with
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent, and without deviating from the objective and scope of technical solution of the present invention, which all should be covered at this
In the middle of the right of invention.
Claims (10)
1. the readable detection means of a kind of immunochromatographic method continuous and quantitative, including upper shell and upper shell cooperatively form accommodation chamber
Lower house and the test strips located at accommodation chamber, the test strips include the pad that liner plate and being located at overlapped on liner plate successively, anti-
Should pad and absorption pad, it is characterised in that：On the reacting pad along pad point to absorption pad direction be sequentially provided with nature controlling line and
Detection zone band, the detection zone band are coated with antigen corresponding with thing to be checked or antibody.
2. the readable detection means of immunochromatographic method continuous and quantitative according to claim 1, it is characterised in that：The upper shell
Be provided with it is corresponding with reacting pad develop the color the observation port of situation for observing detection zone band, the edge of the observation port be provided with
The corresponding concentration scale line for showing measured object content of detection zone band.
3. the readable detection means of immunochromatographic method continuous and quantitative according to claim 2, it is characterised in that：The accommodation chamber
Be additionally provided with sample pad, the rear end of the sample pad is contacted with pad, the upper shell be provided with it is corresponding with sample pad for
Add the well of sample.
4. the readable detection means of immunochromatographic method continuous and quantitative according to claim 3, it is characterised in that：The upper shell
It is provided with the chromatography developing solvent hole for adding developing solvent corresponding with sample pad front end.
5. the readable detection means of immunochromatographic method continuous and quantitative according to claim 3, it is characterised in that：The well
Bottom be provided with sample seperation film, the sample pad is contacted with the lower surface of sample seperation film.
6. the readable detection means of immunochromatographic method continuous and quantitative according to claim 4, it is characterised in that：This device is arranged
There are at least two test strips；The pad of each test strips is contacted with the rear end of sample pad.
7. the readable detection means of immunochromatographic method continuous and quantitative according to any one of claim 3 to 6, it is characterised in that：
Pad is set above described liner plate one end, absorption pad is set above the liner plate other end, with pad below sample pad rear end
Blank end overlaps, and is reacting pad between pad and absorption pad.
8. the readable detection means of immunochromatographic method continuous and quantitative according to any one of claim 1 to 6, it is characterised in that：
The upper shell is hinged with one end of lower house, the other end is connected by buckle.
9. the readable detection means of immunochromatographic method continuous and quantitative according to any one of claim 1 to 6, it is characterised in that：
The reacting pad is cellulose acetate film pad or nitrocellulose membrane pad.
10. the readable detection means of immunochromatographic method continuous and quantitative according to any one of claim 1 to 6, it is characterised in that：
The pad is latex mattress or nanometer gold pad.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611123646.4A CN106526178A (en) | 2016-12-08 | 2016-12-08 | Continuous quantitative readable detection device adopting immunochromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611123646.4A CN106526178A (en) | 2016-12-08 | 2016-12-08 | Continuous quantitative readable detection device adopting immunochromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106526178A true CN106526178A (en) | 2017-03-22 |
Family
ID=58342156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611123646.4A Pending CN106526178A (en) | 2016-12-08 | 2016-12-08 | Continuous quantitative readable detection device adopting immunochromatography |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106526178A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110244035A (en) * | 2019-05-09 | 2019-09-17 | 武汉优恩生物科技有限公司 | The readable detection device of immunochromatographic method continuous and quantitative |
| CN111122856A (en) * | 2020-01-10 | 2020-05-08 | 中国农业科学院烟草研究所 | Time-resolved fluorescence immunochromatography test paper card for detecting butralin |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1165300A (en) * | 1995-10-18 | 1997-11-19 | 株式会社京都第一科学 | Dry test apparatus for glycoalbumin determination |
| CN201035010Y (en) * | 2007-04-20 | 2008-03-12 | 北京万泰生物药业有限公司 | Vertical type immune chromatography test paper strip |
| WO2010105802A2 (en) * | 2009-03-16 | 2010-09-23 | Clondiag Gmbh | Assays |
| CN102565385A (en) * | 2011-12-27 | 2012-07-11 | 深圳市爱速尔生物技术有限公司 | Rapid detection instrument for epidemic antigen and antibody of living animal |
| CN102928589A (en) * | 2012-11-15 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit |
| CN203224492U (en) * | 2013-03-08 | 2013-10-02 | 北京海瑞祥天生物科技有限公司 | Test strip and kit for in-vitro joint inspection and diagnosis |
| CN103364547A (en) * | 2012-04-01 | 2013-10-23 | 嘉善德智医疗器械科技有限公司 | Multi-parameter immunochromatographic assay test paper and preparation method thereof |
| CN104793004A (en) * | 2015-04-24 | 2015-07-22 | 杭州霆科生物科技有限公司 | Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent |
| CN206208904U (en) * | 2016-12-08 | 2017-05-31 | 刘宏川 | The readable detection means of continuous and quantitative |
-
2016
- 2016-12-08 CN CN201611123646.4A patent/CN106526178A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1165300A (en) * | 1995-10-18 | 1997-11-19 | 株式会社京都第一科学 | Dry test apparatus for glycoalbumin determination |
| CN201035010Y (en) * | 2007-04-20 | 2008-03-12 | 北京万泰生物药业有限公司 | Vertical type immune chromatography test paper strip |
| WO2010105802A2 (en) * | 2009-03-16 | 2010-09-23 | Clondiag Gmbh | Assays |
| CN102565385A (en) * | 2011-12-27 | 2012-07-11 | 深圳市爱速尔生物技术有限公司 | Rapid detection instrument for epidemic antigen and antibody of living animal |
| CN103364547A (en) * | 2012-04-01 | 2013-10-23 | 嘉善德智医疗器械科技有限公司 | Multi-parameter immunochromatographic assay test paper and preparation method thereof |
| CN102928589A (en) * | 2012-11-15 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit |
| CN203224492U (en) * | 2013-03-08 | 2013-10-02 | 北京海瑞祥天生物科技有限公司 | Test strip and kit for in-vitro joint inspection and diagnosis |
| CN104793004A (en) * | 2015-04-24 | 2015-07-22 | 杭州霆科生物科技有限公司 | Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent |
| CN206208904U (en) * | 2016-12-08 | 2017-05-31 | 刘宏川 | The readable detection means of continuous and quantitative |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110244035A (en) * | 2019-05-09 | 2019-09-17 | 武汉优恩生物科技有限公司 | The readable detection device of immunochromatographic method continuous and quantitative |
| CN111122856A (en) * | 2020-01-10 | 2020-05-08 | 中国农业科学院烟草研究所 | Time-resolved fluorescence immunochromatography test paper card for detecting butralin |
| CN111122856B (en) * | 2020-01-10 | 2023-05-12 | 中国农业科学院烟草研究所 | Time-resolved fluorescence immunochromatography test paper card for detecting butralin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4225576B2 (en) | Method and apparatus for making changes detectable at the time of verification into a predetermined distribution state | |
| CN101120253B (en) | Immunochromatographic test instrument and semiquantitative method using the same | |
| US7344893B2 (en) | Immuno-gold lateral flow assay | |
| JP4383860B2 (en) | Biosensor and measurement method | |
| US5939252A (en) | Detachable-element assay device | |
| CN104937418B (en) | It is analyzed using the calibration in reaction time | |
| JPH01503174A (en) | Test method | |
| WO2008007359A2 (en) | Rapid diagnostic devices based on molecular imprinted polymers | |
| WO2015184847A1 (en) | Immunochromatographic test method and test paper | |
| JP7241696B2 (en) | Immunochromatographic device | |
| CN105866410A (en) | Immunochromatographic detection test strip for joint detection of NSE and CEA, and preparation method and application method thereof | |
| CN106526178A (en) | Continuous quantitative readable detection device adopting immunochromatography | |
| EP3221444B1 (en) | Lateral flow assay ratio test | |
| CN106645043A (en) | Kit and method for fast quantitatively detecting small molecule compound | |
| CN206208904U (en) | The readable detection means of continuous and quantitative | |
| EP3771908A1 (en) | Lateral-electrophoretic bioassay | |
| CN106645703A (en) | Kit and method for quickly and quantitatively detecting small-molecular compounds | |
| WO2012127122A1 (en) | Method for performing a rapid test | |
| US20220258155A1 (en) | Lateral flow assay with sample adequacy line | |
| CN206740772U (en) | A kind of whole blood is quantitatively loaded the kit of detection cardiac muscle three | |
| CN106645704A (en) | Kit for fast and quantificationally detecting small molecular compound and method | |
| CN106645705A (en) | Kit and method for rapidly and quantitatively detecting small molecule compounds | |
| JPH0317559A (en) | Simple immunodiagnostic device | |
| TW201643431A (en) | Quantitative lateral flow chromatographic structure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170322 |
|
| RJ01 | Rejection of invention patent application after publication |